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Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development15:27

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

We demonstrate the construction of alcohol vapor chambers using readily available materials that simultaneously house 6 mouse cages. We further describe their use in a mouse model of fetal alcohol exposure equivalent to all 3 trimesters of human pregnancy. This paradigm exposes animals during gestation and postnatal days...
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In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines09:57

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Here, we present a highly accessible protocol for evaluating the cell movement in human trophoblast cells using three in vitro assays: the scratch assay, the transwell invasion assay, and the cell proliferation...
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A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots06:12

A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots

This study describes a fast and effective method for the cell component analysis of cerebral blood clots through clot dissolving, cell staining, and routine blood...
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The Comprehensive Infant Examination20:06

The Comprehensive Infant Examination

Source: Heather Collette and Jaideep Talwalkar; Yale School of Medicine
The newborn exam is important in establishing a baby's baseline health status as they enter the world. Newborn providers have the unique opportunity to evaluate a patient for the very first time. They are also tasked with identifying congenital anomalies that require early intervention to promote a long and healthy life for the child. Many newborn anomalies, such as heart defects, are noted on prenatal ultrasound....
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Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits04:29

Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits

This protocol describes a pregnancy and feeding management technique for embryo-transferred and genetically modified rabbits, aimed at reducing newborn rabbit mortality and enhancing the preparation efficiency of gene-edited...
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Transabdominal Ultrasound for Pregnancy Diagnosis in Reeves' Muntjac Deer09:26

Transabdominal Ultrasound for Pregnancy Diagnosis in Reeves' Muntjac Deer

Transabdominal ultrasound is described as an effective, noninvasive means for assessing reproductive status in Reeves' muntjac deer. These methods can be used to achieve early pregnancy diagnosis and to evaluate fetal viability. Future applications of this technique include estimation of gestational age and effects of maternal disease on fetal...
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