Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Harmonic Nanoparticles for Regenerative Research09:23

Harmonic Nanoparticles for Regenerative Research

12.1K
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also...
12.1K
Harmonic Mean01:09

Harmonic Mean

3.6K
The arithmetic mean is usually skewed towards the larger values in the data set. Therefore, to avoid this inherent bias towards smaller values, the harmonic mean is used.
Take the example of the speed of a car, which is the measure of the rate of distance traveled. If the vehicle traverses the same distance back-and-forth, its average speed equals the total distance traveled divided by the total time taken. However, if the car moves with varying speeds, then the arithmetic mean is more skewed...
3.6K
Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects02:40

Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects

381
Source: Piazza, V., et al., Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin. J. Vis. Exp. (2023)This video demonstrates the procedure for imaging microtubule abnormalities in a rat brain tissue slice using a two-photon excitation microscope. It employs second harmonic generation (SHG) signals to detect tubulin defects and reduced myelin...
381
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

584
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
584
Hooke's Law and Simple Harmonic Motion07:52

Hooke's Law and Simple Harmonic Motion

72.9K
Source: Ketron Mitchell-Wynne, PhD, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA
Potential energy is an important concept in physics. Potential energy is the energy associated with forces that depend upon the position of an object relative to its surroundings. Gravitational potential energy, which is discussed in another video, is the energy associated that is directly proportional to the height of an object...
72.9K
Oral Cavity01:11

Oral Cavity

3.0K
The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical Interference Coating Design Challenge 2025: a design for production and an immersed, three-line, polarizing notch filter [Invited].

Applied optics·2026
Same author

Smart color designs with Ag and Au metal island films: a theoretical study.

Applied optics·2026
Same author

Electric-Field Molecular Fingerprinting to Probe Cancer.

ACS central science·2025
Same author

Linear field-resolved spectroscopy approaching ultimate detection sensitivity.

Optics express·2025
Same author

Sub-attosecond-precision optical-waveform stability measurements using electro-optic sampling.

Scientific reports·2024
Same author

Light-wave-controlled Haldane model in monolayer hexagonal boron nitride.

Nature·2024

Related Experiment Video

Updated: Jan 19, 2026

Harmonic Nanoparticles for Regenerative Research
09:23

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

12.1K

Cavity-enhanced noncollinear high-harmonic generation.

Maximilian Högner, Tobias Saule, Stephan Heinrich

    Optics Express
    |September 11, 2019
    PubMed
    Summary
    This summary is machine-generated.

    This study explores using spatiotemporal couplings to improve extreme ultraviolet (XUV) sources, overcoming limitations in efficiency and pulse duration for advanced light generation.

    More Related Videos

    Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects
    02:40

    Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects

    Published on: June 17, 2025

    381
    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
    09:19

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

    Published on: August 29, 2025

    584

    Related Experiment Videos

    Last Updated: Jan 19, 2026

    Harmonic Nanoparticles for Regenerative Research
    09:23

    Harmonic Nanoparticles for Regenerative Research

    Published on: May 1, 2014

    12.1K
    Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects
    02:40

    Second Harmonic Generation Imaging in a Rat Model to Study Tubulin Defects

    Published on: June 17, 2025

    381
    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
    09:19

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

    Published on: August 29, 2025

    584

    Area of Science:

    • Physics
    • Quantum Optics
    • Attosecond Science

    Background:

    • Femtosecond enhancement cavities generate high-repetition-rate coherent extreme ultraviolet (XUV) sources.
    • Current XUV sources face limitations in conversion efficiency and emission duration.

    Purpose of the Study:

    • To investigate harnessing spatiotemporal couplings for improved XUV generation.
    • To circumvent limitations in efficiency and pulse duration of XUV sources.

    Main Methods:

    • Theoretical exploration of spatiotemporal couplings in the driving field.
    • Experimental validation of the proposed method.
    • Utilizing concepts similar to the "attosecond lighthouse".

    Main Results:

    • Predicted dramatically improved output coupling efficiencies.
    • Demonstrated efficient generation of isolated XUV attosecond pulses.
    • Overcame limitations of net conversion efficiency and emission duration.

    Conclusions:

    • Spatiotemporal couplings offer a promising route to enhance XUV source performance.
    • This approach enables efficient generation of isolated attosecond pulses.
    • Potential for advanced applications in ultrafast science.