Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Building a Simple and Versatile Illumination System for Optogenetic Experiments06:41

Building a Simple and Versatile Illumination System for Optogenetic Experiments

4.3K
This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a...
4.3K
Lensless Fluorescent Microscopy on a Chip11:23

Lensless Fluorescent Microscopy on a Chip

18.1K
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and...
18.1K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

10.5K
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction...
10.5K
Three-dimensional Optical-resolution Photoacoustic Microscopy08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

18.8K
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging...
18.8K
Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy09:20

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

11.7K
This paper aims to instruct the reader in the operation of an integrated atomic force-optical imaging microscope for mechanical stimulation of live cells in culture. A step-by-step protocol is presented. A representative data set that shows live cell response to mechanical stimulation is...
11.7K
The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy11:53

The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy

12.2K
We present a protocol on modular design and production of intelligent robots to help scientific and technical workers design intelligent robots with special production tasks based on personal needs and individualized...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient separation of butane isomers via ZIF-8 slurry on laboratory- and pilot-scale.

Nature communications·2022
Same author

Influence of Sea Level Anomaly on Underwater Gravity Gradient Measurements.

Sensors (Basel, Switzerland)·2022
Same author

MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells.

Molecular medicine reports·2022
Same author

Evaluation of Emulsifying Ability of Phospholipids by Langmuir Monolayers and Stability of High Oil Ratio O/W Emulsions.

AAPS PharmSciTech·2022
Same author

Evaluation of Occupational Health and Safety Management of Listed Companies in China's Energy Industry Based on the Combined Weight-Cloud Model: From the Perspective of FPE Information Disclosure.

International journal of environmental research and public health·2022
Same author

Multisystem Langerhans Cell Histiocytosis in Younger Infants First Presenting in Skin: A Case Series.

Journal of personalized medicine·2022

Related Experiment Video

Updated: Jan 19, 2026

Building a Simple and Versatile Illumination System for Optogenetic Experiments
06:41

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

4.3K

VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy.

Bei Liu, Chad M Hobson, Frederico M Pimenta

    Optics Express
    |September 11, 2019
    PubMed
    Summary
    This summary is machine-generated.

    We created VIEW-MOD, a versatile modular microscope supporting multiple illumination methods like light sheet and TIRF. This compact system offers precise control for advanced biological imaging applications.

    More Related Videos

    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.1K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.5K

    Related Experiment Videos

    Last Updated: Jan 19, 2026

    Building a Simple and Versatile Illumination System for Optogenetic Experiments
    06:41

    Building a Simple and Versatile Illumination System for Optogenetic Experiments

    Published on: January 12, 2021

    4.3K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    18.1K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.5K

    Area of Science:

    • Biophotonics and Instrumentation
    • Microscopy and Imaging Technologies
    • Optical Engineering

    Background:

    • Advanced microscopy techniques are crucial for understanding complex biological processes.
    • Existing systems often lack flexibility in illumination and imaging modes, limiting their applicability.
    • The need for adaptable, high-performance imaging solutions in biological research is growing.

    Purpose of the Study:

    • To develop a compact, multi-modality microscope system with flexible illumination control.
    • To enable seamless switching between various illumination schemes and imaging modes.
    • To provide a user-friendly platform for advanced biological imaging applications.

    Main Methods:

    • Development of VIEW-MOD (Versatile Illumination Engine with a Modular Optical Design) microscope.
    • Integration of electrically tunable lenses and fast-steering mirrors for illumination control.
    • Implementation of a modular optical design allowing 6 degrees of freedom for the illumination source.
    • Creation of standalone, GUI-based software for system calibration and control.

    Main Results:

    • VIEW-MOD accommodates variable angle total internal reflection, point scanning, and light sheet illumination.
    • The system offers precise control over illumination source (3D translation, 2D tilt, beam shape) and imaging plane.
    • Demonstrated applications include biosensor imaging, optogenetics, and fast 3D volume imaging.
    • The developed software provides an intuitive interface for microscope operation.

    Conclusions:

    • VIEW-MOD is a versatile and compact multi-modality microscope system.
    • The system's flexible illumination and imaging capabilities address complex imaging needs.
    • It offers broad usability for diverse biological applications requiring precise optical control.