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Cell Imaging Using Total Internal Reflection Fluorescence Microscopy02:51

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Source: Daniele, F., et. al TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis. J. Vis. Exp. (2015).This video demonstrates the imaging of human neuroblastoma cells using a total internal reflection fluorescence (TIRF) microscope to visualize fluorophore-tagged synaptic vesicles. The cells are first focused in epifluorescence mode. Adjustments are then made to achieve total internal reflection,...
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Related Experiment Video

Updated: Jan 19, 2026

Cell Imaging Using Total Internal Reflection Fluorescence Microscopy
02:51

Cell Imaging Using Total Internal Reflection Fluorescence Microscopy

Published on: June 17, 2025

603

Highly angular resolving beam separator based on total internal reflection.

Moritz Mihm, Ortwin Hellmig, André Wenzlawski

    Applied Optics
    |September 11, 2019
    PubMed
    Summary

    This study introduces an optical element that separates beams differing only in angle using total internal reflection. This innovation can reduce laser system size and complexity by shortening beam paths.

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    Area of Science:

    • Optics and Photonics
    • Laser Systems Engineering

    Background:

    • Separating superimposed optical beams with slight angular differences is crucial for various laser applications.
    • Existing methods can be complex and lead to bulky optical setups.

    Purpose of the Study:

    • To develop a novel optical element for efficient angular separation of superimposed beams.
    • To demonstrate the element's effectiveness in a practical application involving acousto-optic modulators.

    Main Methods:

    • Utilizing total internal reflection at an air gap between two prisms to achieve angular resolution.
    • Designing an optical element adaptable to different wavelengths and component sizes.

    Main Results:

    • Successfully demonstrated the separation of superimposed beams with different diffraction orders at 800 nm.
    • The optical element effectively resolves and separates beams based on their angular divergence.

    Conclusions:

    • The presented optical element offers a compact and efficient solution for beam separation based on angle.
    • This technology has the potential to significantly reduce the size and complexity of laser systems.