Related Experiment Video
Updated: Jan 19, 2026
Cell Imaging Using Total Internal Reflection Fluorescence Microscopy
Published on: June 17, 2025
Highly angular resolving beam separator based on total internal reflection
Abstract:
We present an optical element for the separation of superimposed beams that only differ in angle. The beams are angularly resolved and separated by total internal reflection at an air gap between two prisms. As a showcase application, we demonstrate the separation of superimposed beams of different diffraction orders directly behind acousto-optic modulators for an operating wavelength of 800 nm. The wavelength as well as the component size can easily be adapted to meet the requirements of a wide variety of applications. The presented optical element allows one to reduce the lengths of beam paths and thus to decrease laser system size and complexity.
Related Concept Videos
Cell Imaging Using Total Internal Reflection Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy
Total Internal Reflection Fluorescence Microscopy for Visualization of Exocytic Events
06:43Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
10:07Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
14:09High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip