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Circulating microRNA trafficking and regulation: computational principles and practice.

Juan Cui1, Jiang Shu1

  • 1Systems Biology and Biomedical Informatics Laboratory, Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.

Briefings in Bioinformatics
|September 11, 2019
PubMed
Summary

This review explores computational methods for analyzing circulating microRNAs (miRNAs) and their regulation. It offers guidance on designing and analyzing studies of miRNA sorting and targeting for better insights into their biological roles.

Keywords:
RNA interactomeRNA motifcirculating microRNAexosomal microRNAgene regulation networkmicroRNA sortingmicroRNA targetnetwork analysis

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) play crucial roles in intercellular communication.
  • Studies on circulating miRNAs have expanded, revealing their functional significance.
  • Advancements in genomics tools facilitate miRNA research.

Purpose of the Study:

  • To review computational principles for circulating miRNA discovery, sorting, and targeting.
  • To provide practical guidance for designing and analyzing miRNA studies.
  • To survey available bioinformatics methods and tools for miRNA research.

Main Methods:

  • Literature review of computational approaches for miRNA analysis.
  • Survey of informatics tools and methods for miRNA sorting and regulation.
  • Discussion of key computational principles in miRNA discovery.

Main Results:

  • Identified a proliferation of studies and analytical methods for circulating miRNAs.
  • Highlighted new insights into the functional roles of circulating miRNAs.
  • Cataloged various informatics methods, tools, applications, and unsolved problems.

Conclusions:

  • Circulating miRNA research offers new biological insights but requires careful computational analysis.
  • Guidance on study design and analysis is crucial for understanding context-dependent miRNA regulation.
  • The field has significant prospects and broader implications for biological and medical research.