Related Experiment Video
Updated: Jan 19, 2026

Peptide Purification: An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates
Published on: April 30, 2023
Simultaneous Determination of Co-administrated Deflazacort, Aprepitant and Granisetron in Dosage Forms and Spiked
Mahmoud A Tantawy1, Soheir Alweshahy1, Dalia A Elshabasy2
1Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt.
Abstract:
A selective reversed phase high performance liquid chromatography/photodiode array detector (RP-HPLC/PAD) method has been developed for simultaneous determination of the three co-administrated deflazacort, aprepitant and granisetron drugs used with chemotherapy. The three cited drugs have been chromatographed on C18 column using a mobile phase consisting of acetonitrile-0.2% v/v triethylamine (80:20 v/v, pH of 6.6 ± 0.05) with isocratic elution and monitored by photodiode array at 220 nm. International conference on harmonization (ICH) guidelines were followed to validate the developed method. Successful application of the developed method was assessed by the simultaneous determination of the studied drugs in pure forms, dosage forms and plasma samples in the ranges of 0.2-20, 0.4-40 and 0.2-20 μg/mL for deflazacort, aprepitant and granisetron, respectively.
More Related Videos
Related Concept Videos
03:21Peptide Purification: An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates
Dosage Interval and Administration Route: Determination Methods
11:29HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
11:17Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Dosage Regimen: Multiple Oral Dosage
20:23A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

