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Techniques for Imaging Ca2+ Signaling in Human Sperm07:38

Techniques for Imaging Ca2+ Signaling in Human Sperm

Stimulus-evoked [Ca2+]i signals of individual human sperm are assessed. Motile cells are loaded with Ca2+-sensitive fluorescent dye (AM-ester method) and immobilised in a perfusable chamber. Cells are imaged by time-lapse fluorescence microscopy and stimulated via the perfusing medium. Responses of single cells (or regions) are analysed offline using...
17.5K
Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment11:38

Experimental Methods for Efficient Solar Hydrogen Production in Microgravity Environment

Efficient solar-hydrogen production has recently been realized on functionalized semiconductor-electrocatalyst systems in a photoelectrochemical half-cell in microgravity environment at the Bremen Drop Tower. Here, we report the experimental procedures for manufacturing the semiconductor-electrocatalyst device, details of the experimental set-up in the drop capsule and the experimental sequence during free...
8.1K
Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite07:00

Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite

A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this...
7.8K
Fluorimetric Techniques for the Assessment of Sperm Membranes08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also...
14.1K
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System09:28

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.
3.5K
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

The present protocol provides a practical solution that allows for the measurement of apoptosis, mitochondrial membrane potential, and DNA damage in human sperm with a single...
3.3K