Related Experiment Video
Updated: Jan 19, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
Published on: March 5, 2022
Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA
Jin Wang1,2,3, Bing Liang Alvin Chew3,4,5, Yong Lai3
1State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, People's Republic of China.
None:
Chemical modification of transcripts with 5' caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps-m7GpppN, m7GpppNm, GpppN, GpppNm, and m2,2,7GpppG-and 5 'metabolite' caps-NAD, FAD, UDP-Glc, UDP-GlcNAc, and dpCoA. Applying CapQuant to RNA from purified dengue virus, Escherichia coli, yeast, mouse tissues, and human cells, we discovered new cap structures in humans and mice (FAD, UDP-Glc, UDP-GlcNAc, and m7Gpppm6A), cell- and tissue-specific variations in cap methylation, and high proportions of caps lacking 2'-O-methylation (m7Gpppm6A in mammals, m7GpppA in dengue virus). While substantial Dimroth-induced loss of m1A and m1Am arose with specific RNA processing conditions, human lymphoblast cells showed no detectable m1A or m1Am in caps. CapQuant accurately captured the preference for purine nucleotides at eukaryotic transcription start sites and the correlation between metabolite levels and metabolite caps.
More Related Videos
13:58Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
07:27Author Spotlight: On-Site Biochar Production for Woody Debris Incineration in Forestry
Published on: January 5, 2024
Related Concept Videos
14:40Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
13:58Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
07:27Producing, Characterizing and Quantifying Biochar in the Woods Using Portable Flame Cap Kilns
11:00Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
10:40Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
09:35Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis