Related Concept Videos

A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model02:26

A Technique to Generate a Human PBMC-Engrafted Humanized Xenograft Mouse Model

This video demonstrates an assay to generate a humanized xenograft mouse model. An immunocompromised mouse is injected with cyclophosphamide to cause immune cell depletion. Patient-derived tumor cells mixed with human peripheral blood mononuclear cells (PBMCs) in a hydrogel are subcutaneously injected into the mouse, forming a tumor with infiltrating T...
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Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma10:52

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying...
11.6K
Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues02:35

Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues

The present protocol describes the method for establishing a patient-derived xenograft (PDX) mouse model using human osteosarcoma...
1.5K
Xenograft Skin Model to Manipulate Human Immune Responses In Vivo08:23

Xenograft Skin Model to Manipulate Human Immune Responses In Vivo

The present protocol describes how to graft human skin onto non-obese diabetic (NOD)-scid interleukin-2 gamma chain receptor (NSG) mice. A detailed description of the preparation of human skin for transplant, preparation of mice for transplant, transplantation of split-thickness human skin, and post-transplantation recovery procedure are included in the report.
3.7K
Isolating Nasal Olfactory Stem Cells from Rodents or Humans09:19

Isolating Nasal Olfactory Stem Cells from Rodents or Humans

We describe here a method for biopsying olfactory mucosa from rat and human nasal cavities. These biopsies can be used for either identifying molecular anomalies in brain diseases or isolating multipotent adult stem cells that can be utilized for cell transplantation in animal models of brain...
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Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice07:48

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

Complex human diseases can be challenging to model in traditional laboratory model systems. Here, we describe a surgical approach to model human muscle disease through the transplantation of human skeletal muscle biopsies into immunodeficient...
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