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Updated: Jan 19, 2026
Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
Different DNA methylation profile is demonstrated in paracoccidioidomycosis patients without oral lesions
Paola Singi1, Raissa P Rocha2, Marina L de Carli1
1Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, Alfenas, MG, Brazil.
Background:
Paracoccidioidomycosis (PCM) is a neglected fungal infection with a high impact on the quality of life of the affected patients. The disease presents primary pulmonary involvement and systemic dissemination may occur. About 50% of the cases show oral involvement, and the factors that lead to this manifestation are not clear.
Objectives:
The aim of this study was to evaluate the DNA methylation profile in PCM patients with oral lesions.
Material And Methods:
Genomic DNA was extracted from whole blood of eighteen PCM patients, being ten with oral lesions and eight with no oral lesion. Analysis of methylation profile was performed using the technique of methylation-sensitive arbitrarily primed PCR (MS-AP-PCR). The sequences of recombinant plasmids obtained were evaluated according to parameters that define a CpG island, as well as their relative position in the known human genome genes and/or CpG islands.
Results:
After DNA amplification, three different expressed bands were observed between the two groups, being found in the samples of patients with no oral manifestations. The cloned fragment in the plasmid showed similarity with a DNA sequence present in chromosome 20, next to the YTHDF1 gene. Other bands showed homology with intronic region in the genes RBPMS2 and DPH6 and no CpG island was identified.
Conclusions:
DNA methylation was found in PCM patients with no oral lesion affecting the YTHDF1 gene. Further studies are necessary to elucidate to role of YTHDF1 gene in the oral PCM manifestations.
Insights
DNA methylation was identified in patients with paracoccidioidomycosis (PCM) but no oral lesions, specifically impacting the YTHDF1 gene. Further research is needed to understand YTHDF1
Area of Science:
- Mycology
- Genetics
- Epigenetics
Background:
- Paracoccidioidomycosis (PCM) is a neglected fungal infection impacting quality of life.
- PCM primarily affects the lungs, with potential for systemic spread.
- Oral involvement occurs in about 50% of cases, but causative factors remain unclear.
Purpose of the Study:
- To investigate the DNA methylation profile in patients with paracoccidioidomycosis (PCM).
- To specifically compare methylation patterns between PCM patients with and without oral lesions.
Main Methods:
- Genomic DNA was extracted from whole blood of 18 PCM patients (10 with oral lesions, 8 without).
- Methylation profiles were analyzed using methylation-sensitive arbitrarily primed PCR (MS-AP-PCR).
- Amplified DNA sequences were analyzed for CpG island characteristics and genomic location.
Main Results:
- Three distinct amplified DNA bands were observed, present only in PCM patients without oral lesions.
- One fragment showed similarity to a DNA sequence on chromosome 20 near the YTHDF1 gene.
- Other fragments were homologous to intronic regions of RBPMS2 and DPH6 genes; no CpG islands were identified.
Conclusions:
- DNA methylation was detected in PCM patients lacking oral lesions, affecting the YTHDF1 gene.
- The findings suggest a potential role for YTHDF1 in the pathogenesis of oral PCM.
- Further investigation is required to clarify the specific role of YTHDF1 in oral manifestations of PCM.
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