Related Experiment Video
Updated: Aug 8, 2026

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
Published on: April 20, 2017
The synthesis of peptides in enteroendocrine cells developing in explanted presumptive endoderm
J Drukker1, E A Terwindt-Rouwenhorst, E L Wiertz-Hoessels
1Department of Anatomy and Embryology, University of Limburg, Maastricht, The Netherlands.
Abstract:
Peptide immunoreactivity was studied in grafts of endoderm from chick or quail embryos (19 hours, corresponding to stage 4- to 5 according to Hamburger and Hamilton) in the coelom of a 48 hour host embryo. The presence of gastrin, glucagon, cholecystokinin, VIP, substance P, somatostatin, bombesin, motilin, secretin, pancreatic polypeptide, neurotensin and insulin was demonstrated. The nature of the peptide(s) generally matched the regional differentiation of the enteric epithelium and the underlying mesodermal components. The findings are compatible with the concept of heterogeneity of the endoderm due to migration of epiblast derived cells.
Related Concept Videos
Protein Digestion
Hormonal Regulation
Insulin Secretory Vesicles
Renewal of Intestinal Stem Cells
Cells and Secretions of the Pancreas
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
Hormones Secreted by the Stomach
Each of these hormones secreted by different enteroendocrine cells plays a unique role in digestion. Here are a few examples:

