Post-Traumatic Stress Disorder Chronification via Monoaminooxidase and Cortisol Metabolism

Post-traumatic stress disorder (PTSD) is a psychiatric condition that arises following exposure to traumatic events such as natural disasters, forced displacement, or severe accidents. It significantly impairs individuals' ability to cope with daily activities and disrupts their emotional and psychological equilibrium.
Symptoms and Behavioral Manifestations
A spectrum of distressing symptoms characterizes PTSD. Recurrent flashbacks, where individuals involuntarily relive traumatic events,...

Source: Tendler, A., et al. How to Use the H1 Deep Transcranial Magnetic Stimulation Coil for Conditions Other than Depression. J. Vis. Exp. (2017)This video demonstrates the use of deep transcranial magnetic stimulation (dTMS) to alleviate symptoms of post-traumatic stress disorder, or PTSD. It begins by identifying reduced activity in the medial prefrontal cortex (mPFC) as a key factor contributing to the overactivation of the amygdala and heightened fear responses. By applying targeted...

Here we describe the detailed methodology required to conduct stress-enhanced fear learning (SEFL) experiments, a preclinical model of post-traumatic stress disorder, in both rats and mice. The model utilizes aspects of Pavlovian fear conditioning and freezing as an index of enhanced fear in rodents.

Recently, it has been discovered that behavioral conditions such as, depression, anxiety and stress-response have a neurological basis. Understanding the biological underpinnings of these conditions may help scientists in developing more effective therapies to treat these disorders. Typically, rodent models are used in this field and behavioral scientists create these models using interventions like drug administration or surgery. It is important to understand how to create and evaluate rodent...

Cortisol (CORT) accumulates in the growing hair shaft of humans and nonhuman primates. We describe methods for extracting and analyzing hair CORT with high precision and sensitivity. Measurement of hair CORT is particularly well-suited for assessing chronic stress over periods of weeks to...

This systematic protocol describes a new animal model of post-traumatic epilepsy after repetitive mild traumatic brain injury. The first part details steps for traumatic brain injury induction using a modified weight drop model. The second part provides instructions on the surgical approach for single- and multi-channel electroencephalographic data acquisition...