Related Experiment Video

Updated: Jan 19, 2026

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

11.6K

[Clinical implications of the crosstalk between islet endothelial cells and β cells]

Y Y Le1, J Yang, T P Hong

  • 1Department of Endocrinology and Metabolism, Peking University Third Hospital, Beijing 100191, China.

Zhonghua Yi Xue Za Zhi
|September 11, 2019
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices05:51

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

Published on: April 12, 2021

4.6K
Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres09:31

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Published on: November 5, 2016

7.7K

Related Concept Videos

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Critical challenges for the diabetes research field are to understand the molecular mechanisms that regulate islet β-cell replication and to develop methods for stimulating β-cell regeneration. Herein a high-content screening method to identify and assess the β-cell replication-promoting activity of small molecules is...
11.6K
Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices05:51

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

This study presents the application of live pancreatic tissue slices to the study of islet physiology and islet-immune cell interactions.
4.6K
Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres09:31

Sustained Administration of β-cell Mitogens to Intact Mouse Islets Ex Vivo Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Here, we present methodology to generate and administer compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres to intact mouse islets in culture with subsequent immunofluorescence analysis of β-cell proliferation. This method is suitable for determining the efficacy of candidate β-cell...
7.7K
Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets08:50

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Beta-cell functionality is important for blood-glucose homeostasis, which is evaluated at single-cell resolution using a genetically encoded reporter for calcium...
15.3K
Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells08:03

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is...
8.8K
Harvest and Primary Culture of Leptomeningeal Lymphatic Endothelial Cells06:44

Harvest and Primary Culture of Leptomeningeal Lymphatic Endothelial Cells

Leptomeningeal lymphatic endothelial cells (LLECs), a recently identified intracranial cell type, have poorly understood functions. This study presents a reproducible protocol for harvesting LLECs from mice and establishing in vitro primary cultures. This protocol is designed to enable researchers to delve into the cellular functions and potential clinical implications of...
2.3K