[Correlation between Mycoplasma pneumoniae DNA replication level and disease severity in children with severe Mycoplasma pneumoniae pneumonia]
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Updated: Jan 19, 2026
Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Ke-Nan Fang1, Jing Wang, Jing-Wen Ni
1Pediatric Intensive Care Unit, Women and Children's Health Care Center of Luoyang, Luoyang, Henan 471000. 328414395@qq.com.
Objective:
To study the correlation of Mycoplasma pneumoniae DNA (MP-DNA) replication level in throat swab and bronchoalveolar lavage fluid (BALF) with disease severity in children with severe Mycoplasma pneumoniae pneumonia (SMPP).
Methods:
A total of 44 children with SMPP who underwent bronchoalveolar lavage were enrolled as subjects. The serum levels of cytokines and MP-DNA replication times in throat swab were measured in the acute stage and the recovery stage, and the levels of interleukin (IL)-8 and MP-DNA replication times in BALF were measured in the acute stage. According to whether mechanical ventilation was needed for respiratory failure, the children were divided into a mechanical ventilation group (n=19) and a non-mechanical ventilation group (n=25), and the two groups were compared in MP-DNA replication times in BALF.
Results:
For the children with SMPP, serum levels of C-reactive protein, erythrocyte sedimentation rate, lactate dehydrogenase, IL-1, IL-6, IL-8, and IL-18 in the acute stage were significantly higher than those in the recovery stage (P<0.05). In the acute stage, MP-DNA replication times in throat swab were positively correlated with those in BALF (r=0.613, P<0.05), and MP-DNA replication times in BALF were positively correlated with IL-18 levels in peripheral blood and BALF (r=0.613 and 0.41 respectively, P<0.05). Compared with the non-mechanical ventilation group, the mechanical ventilation group had significantly higher MP-DNA replication times in BALF, a significantly longer duration of systemic hormone treatment, significantly higher serum levels of lactate dehydrogenase and IL-18, and significantly higher white blood cell count and IL-18 level in BALF (P<0.05).
Conclusions:
In children with SMPP, MP-DNA replication level in throat swab and BALF can be used as a reference index for the assessment of disease severity.
Insights
Mycoplasma pneumoniae DNA (MP-DNA) replication levels in throat swabs and bronchoalveolar lavage fluid (BALF) correlate with disease severity in children with severe Mycoplasma pneumoniae pneumonia (SMPP). Higher MP-DNA replication in BALF indicates more severe disease, suggesting its use as a severity assessment index.
Area of Science:
- Pediatric Infectious Diseases
- Respiratory Medicine
- Molecular Diagnostics
Background:
- Severe Mycoplasma pneumoniae pneumonia (SMPP) poses a significant threat to children's respiratory health.
- Accurate assessment of SMPP severity is crucial for timely and effective clinical management.
- Understanding the role of Mycoplasma pneumoniae DNA (MP-DNA) replication in disease progression is essential.
Purpose of the Study:
- To investigate the correlation between MP-DNA replication levels in throat swabs and bronchoalveolar lavage fluid (BALF).
- To determine the association of MP-DNA replication with disease severity in children diagnosed with SMPP.
- To evaluate MP-DNA replication as a potential biomarker for SMPP severity.
Main Methods:
- A cohort of 44 children with SMPP undergoing BAL was studied.
- MP-DNA replication levels in throat swabs and BALF were quantified during acute and recovery stages.
- Cytokine levels (including IL-8) and clinical data (e.g., need for mechanical ventilation) were analyzed.
Main Results:
- MP-DNA replication in throat swabs positively correlated with that in BALF during the acute stage.
- Higher MP-DNA replication levels in BALF were significantly associated with increased disease severity, indicated by the need for mechanical ventilation.
- Elevated MP-DNA replication in BALF also correlated with higher levels of IL-18 in both peripheral blood and BALF.
Conclusions:
- MP-DNA replication levels in both throat swabs and BALF serve as valuable reference indices for assessing SMPP severity in children.
- These findings support the potential use of MP-DNA quantification as a diagnostic and prognostic tool in pediatric pneumonia.
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