Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing

Area of Science:

• Molecular Biology
• Genomics
• Oncology

Background:

• Colorectal adenoma detection is crucial for preventing colorectal cancer.
• Circulating mRNA markers offer a minimally invasive approach for early detection.
• Previous work optimized plasma targeted mRNA sequencing protocols.

Purpose of the Study:

• To identify potential circulating mRNA biomarkers for colorectal adenoma.
• To compare plasma mRNA profiles between colorectal adenoma patients and healthy controls.

Main Methods:

• Plasma targeted mRNA sequencing was performed on 40 colorectal adenoma patients and 39 healthy controls.
• Differential gene expression analysis was conducted using a fold change >2 and adjusted P value <0.05 cutoff.
• Enrichment analysis identified key biological processes associated with adenoma formation.

Main Results:

• GSK3A and RHOA were identified as differentially expressed genes.
• Both GSK3A (0.01-fold) and RHOA (0.35-fold) showed significantly lower expression in adenoma patients compared to controls.
• Key biological processes implicated in adenoma formation include regulation of programmed cell death and cell differentiation.

Conclusions:

• GSK3A and RHOA are potential circulating mRNA markers for colorectal adenoma.
• The findings contribute to understanding the molecular mechanisms underlying adenoma development.
• This research highlights the utility of plasma mRNA sequencing for non-invasive disease detection.