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Oral Biofilm Formation on Different Materials for Dental Implants11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Here, we present a protocol to evaluate oral biofilm formation on titanium and zirconia materials for dental prosthesis abutments, including the analysis of bacterial cells viability and morphological characteristics. An in situ model associated with powerful microscopy techniques is used for the oral biofilm...
12.1K
A Standardized Approach to Extra-Oral and Intra-Oral Digital Photography06:49

A Standardized Approach to Extra-Oral and Intra-Oral Digital Photography

The present protocol describes a method to capture intra- and extra-oral photographs for digital documentation in the clinical practice of dentistry. The protocol highlights all the critical components involved and the commonly utilized settings to capture dental photographs for clinical documentation.
10.0K
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Changes in the oral microbiome throughout childhood are of growing interest. Comparison of different microbiome studies reveals a lack of standardized sampling protocols. Limited space makes sampling the sound subgingival sulcus of children challenging. Paper point sampling is presented here in detail as the method of choice for this...
17.8K
Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models06:16

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Microhardness is a mechanical property and an informative parameter for evaluating hard tissue pathophysiology. Here, we demonstrate a standardized protocol (sample preparation, polishing, flat surface, and indentation sites) for microhardness analysis in tooth and alveolar bone in rodent oral disease models, namely, dental fluorosis, and ligature-induced periodontal bone...
1.2K
Oral Health Assessment by Lay Personnel for Older Adults08:47

Oral Health Assessment by Lay Personnel for Older Adults

The goal of this protocol is to enable non-dental professionals to assess oral health status for research or health-screening purposes. Aspects assessed include lips, tongue, soft and hard tissues, natural and artificial teeth, oral cleanliness, plaque, swallowing, and impact of oral health on quality of...
13.5K
Primary Culture of Dental Pulp Stem Cells03:45

Primary Culture of Dental Pulp Stem Cells

This article provides a stepwise guide to establish a primary culture of dental pulps stem cells using the explant culture method and characterization of these cells based on ICSCRT guidelines. The cells isolated by this protocol can be considered as mesenchymal stem cells for further...
3.2K