Pregnane glycosides from Gymnema inodorum and their α-glucosidase inhibitory activity

Do Thi Trang1, Duong Thi Hai Yen1, Nguyen The Cuong2

  • 1Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.

Natural Product Research
|September 12, 2019
PubMed

Related Concept Videos

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity03:20

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity

This video demonstrates the in vitro measurement of enzyme activity for α-galactosidase A and acid α-glucosidase in samples using synthetic 4-Methylumbelliferyl...
604
Oral Hypoglycemic Agents: α-Glucosidase Inhibitors01:19

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are...
532
Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities09:10

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good...
7.1K
Evaluating Mutation Frequency in Bacteria Using the Beta-Glucosidase Assay04:00

Evaluating Mutation Frequency in Bacteria Using the Beta-Glucosidase Assay

Source: Stefan, A., et al. The Multifaceted Benefits of Protein Co-expression in Escherichia coli. J. Vis. Exp. (96), (2015).This video demonstrates the evaluation of mutation frequency in bacteria using a beta-glucosidase assay. Bacterial cultures expressing a proofreading-deficient DNA polymerase are collected over successive generations. Replication errors caused by reduced proofreading may activate a suppressed beta-glucosidase gene. After centrifugation and permeabilization, a substrate is...
178
Detecting Neurodegenerative Disease-Associated Protein α-Synuclein Using ELISA04:37

Detecting Neurodegenerative Disease-Associated Protein α-Synuclein Using ELISA

Source: Bétemps, D., et al.,  Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein. J. Vis. Exp. (2015).This video demonstrates the procedure to detect neurodegenerative disease-associated protein α-synuclein in brain tissue from transgenic mice using ELISA. The steps include tissue homogenization, centrifugation, and ELISA detection of α-synuclein protein to quantify...
539
Liquid Phase Isoelectric Focusing: An Electrophoresis Technique to Separate Bioactive Molecules from Crude Gymnema sylvestre Extract03:11

Liquid Phase Isoelectric Focusing: An Electrophoresis Technique to Separate Bioactive Molecules from Crude Gymnema sylvestre Extract

This video describes a technique to separate complex molecules from plant extracts using liquid phase isoelectric focusing. This technique helps purify complex biomolecules in their native state which can be used for various downstream...
1.5K