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Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis08:52

Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis

Nanoparticle tracking analysis (NTA) is a widely used method to characterize extracellular vesicles. This paper highlights NTA experimental parameters and controls plus a uniform method of analysis and characterization of samples and diluents necessary to supplement the guidelines proposed by MISEV2018 and EV-TRACK for reproducibility between...
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Induction and Assessment of Exertional Skeletal Muscle Damage in Humans08:33

Induction and Assessment of Exertional Skeletal Muscle Damage in Humans

This article describes a safe and reliable method to induce and quantify exertional skeletal muscle damage in human...
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Efficiently Recording the Eye-Hand Coordination to Incoordination Spectrum07:30

Efficiently Recording the Eye-Hand Coordination to Incoordination Spectrum

Cerebral injury can damage both ocular and somatic motor systems. Characterization of motor control post-injury affords biomarkers that assist in disease detection, monitoring, and prognosis. We review a method to measure eye-hand movement control in health and in pathologic incoordination, with look-and-reach paradigms to assess coordination between eye and...
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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

In many biological and clinical situations it is advantageous to study cellular processes as they evolve in their native microenvironment. Here we describe the assembly and use of a low-cost fiber-optic microscope which can provide real time imaging in cell culture, animal studies, and clinical patient...
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Identification of Fatty Acids in Bacillus cereus08:41

Identification of Fatty Acids in Bacillus cereus

We propose a protocol to identify fatty acids without the need to purify them. It combines information on the retention times with the mass spectra of three types of fatty acid derivatives: fatty acid methyl esters (FAMEs), 4,4-dimethyl oxazoline derivatives (DMOX), and 3-pyridylcarbinyl esters (picolinyl).
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Walk with Me Hybrid Virtual/In-Person Walking for Older Adults with Neurodegenerative Disease07:21

Walk with Me Hybrid Virtual/In-Person Walking for Older Adults with Neurodegenerative Disease

The present protocol aims to inform rehabilitation specialists and fitness instructors in safe, feasible, and evidence-based methods of delivering virtual and in-person walking classes to older adults with neurodegenerative diseases.
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