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Culturing Microglia from the Neonatal and Adult Central Nervous System11:28

Culturing Microglia from the Neonatal and Adult Central Nervous System

We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial...
28.8K
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System14:55

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to...
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Generation of a Murine Model with a Central Nervous System Infection02:21

Generation of a Murine Model with a Central Nervous System Infection

This video demonstrates the generation of a mouse model with a CNS infection. A suckling pup is injected with the virus both intracranially and intraperitoneally. The virus spreads, infecting the CNS causing neuronal cell death and inflammation. The pup is assessed for symptoms of infection as the disease...
494
The Establishment and Utilization of Patient Derived Xenograft Models of Central Nervous System Metastasis06:52

The Establishment and Utilization of Patient Derived Xenograft Models of Central Nervous System Metastasis

Central nervous system metastasis PDX models represent the phenotypic and molecular characteristics of human metastasis, making them excellent models for preclinical studies. Described here is how to establish PDX models and the inoculation routes that are best used for preclinical...
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Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY10:28

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive...
14.0K
Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE20:51

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

In this video we demonstrate how to isolate mononuclear cells from the central nervous system of rats with experimental autoimmune encephalomyelitis.
16.7K