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Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels08:37

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels

Here, we present a protocol to establish a long-term liver cold storage and transplantation model in Daurian ground squirrel (DGS), which has been technically challenging in standard rodent models.
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New Thrombectomy Technique for Total Portal Vein Thrombosis in Liver Transplantation08:45

New Thrombectomy Technique for Total Portal Vein Thrombosis in Liver Transplantation

Portal vein thrombosis (PVT) is a frequent complication in patients with portal hypertension resulting from end-stage liver disease. In certain instances, PVT may be a contraindication for liver transplantation. We have developed a novel approach for managing PVT cases involving complete occlusion of the main trunk of the portal...
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Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation05:25

Preservation of Porcine Donation after Circulatory Death (DCD) Liver by Perfusion and Orthotopic Liver Transplantation

This protocol outlines the methodology of establishing a porcine model utilizing variable temperature-controlled Machine Perfusion (MP) for the preservation of the donor liver, followed by orthotopic liver transplantation (OLTx). It aims to promote the success rate of OLTx using donor donation after circulatory death (DCD) liver and establish a stable...
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Manufacturing Devices and Instruments for Easier Rat Liver Transplantation08:02

Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

We describe the design of the “quick-linker” device for easier orthotopic rat liver...
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Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation08:39

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

The goal of this study is to modify the rat orthotopic liver transplant model to better represent human liver transplantation and improve recipient survival. The presented method reestablishes hepatic arterial inflow by connecting the donor liver's common hepatic artery to the recipient liver's proper hepatic...
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Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography09:23

Successful Orthotopic Liver Transplantation in Mice Utilizing Microcomputed Tomography Angiography

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.
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