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Related Concept Videos

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

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A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron...
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Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells09:18

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

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A correlative light electron microscopy (CLEM) method is applied to image virus-induced intracellular structures via electron microscopy (EM) in cells that are previously selected by light microscopy (LM). LM and EM are combined as a hybrid imaging approach to achieve an integrated view of virus-host...
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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy09:21

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We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron...
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Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy11:53

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Here, we present a protocol for optimized on-section correlative light-electron microscopy based on endogenous, fluorescent labeling as a tool to investigate the localization of rare proteins in relation to cellular ultrastructure. The power of this approach is demonstrated by ultrastructural localization of endogenous LC3 in starved cells without Bafilomycin treatment.
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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

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This protocol describes a mechanism for using correlative light and electron microscopy to visualize the interaction of mitochondria and lysosomes labeled with mEosEM and APEX2,...
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Epon Post Embedding Correlative Light and Electron Microscopy08:47

Epon Post Embedding Correlative Light and Electron Microscopy

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We present a detailed protocol for Epon post-embedding correlative light and electron microscopy using a fluorescent protein called mScarlet. This method can maintain the fluorescence and the ultrastructure simultaneously. This technique is amenable to a wide variety of biological...
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Related Experiment Video

Updated: Jan 19, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

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Volume-CLEM: a method for correlative light and electron microscopy in three dimensions.

Jan Hegermann1,2, Christoph Wrede1,2, Susanne Fassbender1

  • 1Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.

American Journal of Physiology. Lung Cellular and Molecular Physiology
|September 12, 2019
PubMed
Summary

Researchers developed a workflow for correlative light and electron microscopy (LM and EM) on the same tissue sections. This method enables 3D reconstruction for identifying rare cellular structures across various organs.

Keywords:
correlative light and electron microscopyfluorescence microscopyfocused ion beam scanning electron microscopylungtissue clearing

More Related Videos

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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Related Experiment Videos

Last Updated: Jan 19, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

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Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
09:18

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
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Published on: July 20, 2019

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Area of Science:

  • Biological imaging
  • Microscopy techniques
  • Cellular and tissue analysis

Background:

  • Three-dimensional (3D) data generation from serial tissue sections using light microscopy (LM) aids in identifying rare structures.
  • Correlative microscopy combines different imaging modalities to leverage their complementary strengths.

Purpose of the Study:

  • To present a workflow for correlative light and electron microscopy (LM-EM) on identical sectioned material.
  • To enable sequential 3D LM and 3D EM analysis from the same samples.

Main Methods:

  • Serial sectioning of biological tissues.
  • Imaging of sections using light microscopy (LM) for 3D reconstruction.
  • Subsequent imaging of the same sections using electron microscopy (EM) for high-resolution 3D analysis.
  • Correlative workflow from 3D LM to 3D EM.

Main Results:

  • Demonstration of a reproducible workflow for correlative 3D LM-EM.
  • Successful application of the workflow to various organs.
  • Facilitation of rare structure identification through combined morphological and fluorescent labeling data.

Conclusions:

  • The presented LM-EM workflow is practical for routine electron microscopy laboratories.
  • This approach enhances the ability to study cellular and tissue architecture in three dimensions.
  • It is applicable to diverse research questions requiring high-resolution morphological data.