Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

7.1K
This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar...
7.1K
A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring05:54

A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring

2.1K
This protocol will explain how to establish a hypertrophic scarring murine model that increases mechanotransduction signaling to simulate human-like scarring. This method involves increasing mechanical tension across a healing incision in a mouse and using a specialized device to create reproducible, excessive scar tissue for detailed histological and bioinformatic...
2.1K
Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay07:40

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

3.3K
This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar...
3.3K
Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model03:45

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

4.2K
Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this...
4.2K
Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain06:44

Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain

20.3K
A demonstration of the bedside test for cutaneous allodynia and its clinical...
20.3K
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function11:35

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

16.9K
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular...
16.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Matrix stiffness induced CREB3L1 activation contributed to skin fibrosis through calcium influx triggered endoplasmic reticulum stress.

Cellular and molecular life sciences : CMLS·2026
Same author

DLP bioprinting of cartilage organoid-laden bioinks yields high-fidelity auricular constructs with enhanced chondrogenesis.

Stem cell research & therapy·2026
Same author

4D-aware stereo matching via implicit spectral reconstruction with multi-modal training and RGB-only deployment.

Optics express·2026
Same author

Multifunctional scaffold inspired by hepatocyte exosomes promotes bone regeneration by regulating osteogenic differentiation via PI3K/AKT pathway.

Materials today. Bio·2026
Same author

Replication-stress-induced chromatin loops protect fork stability.

Nature·2026
Same author

Weakly Textured Objects Pose Estimation: A Comprehensive Review.

Sensors (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jan 19, 2026

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

7.1K

Experimental models for cutaneous hypertrophic scar research.

Jialun Li1, Jiecong Wang1, Zhenxing Wang1

  • 1Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society
|September 12, 2019
PubMed
Summary

Understanding hypertrophic scar models is key to preventing abnormal wound repair. This review details in vivo and in vitro models for scar research and treatment development.

More Related Videos

A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring
05:54

A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring

Published on: November 29, 2024

2.1K
Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay
07:40

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

Published on: April 28, 2022

3.3K

Related Experiment Videos

Last Updated: Jan 19, 2026

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

7.1K
A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring
05:54

A Mouse Model of Mechanotransduction-driven, Human-like Hypertrophic Scarring

Published on: November 29, 2024

2.1K
Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay
07:40

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay

Published on: April 28, 2022

3.3K

Area of Science:

  • Dermatology and Regenerative Medicine
  • Wound Healing Research
  • Biomedical Engineering

Background:

  • Human skin wound repair frequently results in scar formation, with hypertrophic scars representing abnormal healing phenotypes.
  • Investigating the mechanisms of scar formation is crucial for developing effective anti-scarring treatments.
  • Numerous in vivo and in vitro models exist to study cutaneous scarring.

Purpose of the Study:

  • To review and describe existing in vivo and in vitro models for studying hypertrophic scars.
  • To highlight the distinct characteristics and applications of each model type.
  • To guide researchers in selecting appropriate models for their specific study goals.

Main Methods:

  • Comprehensive literature review of established in vivo (animal) and in vitro (cell-based) models for hypertrophic scar research.
  • Analysis of the strengths and limitations of each model type in relation to scar formation mechanisms and treatment development.
  • Categorization of models based on their suitability for phenotypical analysis, pathway identification, and drug screening.

Main Results:

  • In vivo models are generally suited for phenotypical scar analysis, anti-scarring treatment development, and gene function studies.
  • In vitro models are advantageous for identifying scar formation pathways and high-throughput drug screening.
  • Each model possesses unique characteristics influencing its applicability in different research contexts.

Conclusions:

  • The selection of an appropriate hypertrophic scar model is contingent upon the specific research objectives and desired outcomes.
  • Understanding the nuances of current models is essential for improving existing methodologies and developing novel research tools.
  • Advancements in hypertrophic scar modeling will significantly contribute to the progress of wound healing and anti-scarring therapies.