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Updated: Jan 19, 2026

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
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Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

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Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9

Takahiko Matsuda1, Izumi Oinuma1,2

  • 1Laboratory of Cell and Molecular Biology, Graduate School of Life Science, University of Hyogo, Hyogo 678-1297, Japan.

Molecular Biology of the Cell
|September 12, 2019
PubMed
Summary

Researchers developed a nonviral CRISPR/Cas9 method to tag proteins in single neurons. This technique allows for precise visualization of protein localization and dynamics in neural networks without altering native expression levels.

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