Transcriptome Reveals the Rice Response to Elevated Free Air CO₂ Concentration and TiO₂ Nanoparticles

Increasing CO₂ levels are speculated to change the effects of engineered nanomaterials in soil and on plant growth. How plants will respond to a combination of elevated CO₂ and nanomaterials stress has rarely been investigated, and the underlying mechanism remains largely unknown. Here, we conducted a field experiment to investigate the rice (Oryza sativa L. cv. IIyou) response to TiO₂ nanoparticles (nano-TiO₂, 0 and 200 mg kg^-1) using a free-air CO₂ enrichment system with different CO₂ levels (ambient ∼370 μmol mol^-1 and elevated ∼570 μmol mol^-1). The results showed that elevated CO₂ or nano-TiO₂ alone did not significantly affect rice chlorophyll content and antioxidant enzyme activities. However, in the presence of nano-TiO₂, elevated CO₂ significantly enhanced the rice height, shoot biomass, and panicle biomass (by 9.4%, 12.8%, and 15.8%, respectively). Furthermore, the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that genes involved in photosynthesis were up-regulated while most genes associated with secondary metabolite biosynthesis were down-regulated in combination-treated rice. This indicated that elevated CO₂ and nano-TiO₂ might stimulate rice growth by adjusting resource allocation between photosynthesis and metabolism. This study provides novel insights into rice responses to increasing contamination under climate change.