Driving Forces of Liquid-Liquid Phase Separation in Biological Systems

Boris Y Zaslavsky1, Luisa A Ferreira2, Vladimir N Uversky3,4

  • 1Cleveland Diagnostics, Cleveland, OH 44114, USA. Boris.Zaslavsky@Cleveland-Diagnostics.com.

Biomolecules
|September 13, 2019
PubMed

Related Concept Videos

Efficiency of Liquid-liquid Extraction09:21

Efficiency of Liquid-liquid Extraction

Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA
Liquid-liquid extraction (LLE) is a separation technique used instead of distillation when either: (a) the relative volatilities of the compounds to be separated are very similar; (b) one or more of the mixture components are temperature sensitive even near ambient conditions; (c) the distillation would require a very low pressure or a very high distillate/feed...
49.9K
In situ TEM of Biological Assemblies in Liquid08:28

In situ TEM of Biological Assemblies in Liquid

Here we describe a procedure to image viral complexes in liquid at nanometer resolution using a transmission electron...
10.6K
Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers12:37

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers

Here, we present a protocol to investigate multi-component phase diagrams using externally controlled magnetic beads as liquid carriers in a lab-in-tube approach. This approach can aid in applications that seek to gather further information on phase change in complex liquid...
12.9K
Liquid Phase Reactor: Sucrose Inversion11:00

Liquid Phase Reactor: Sucrose Inversion

Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA
Both batch and continuous flow reactors are used in catalytic reactions. Packed beds, which use solid catalysts and a continuous flow, are the most common configuration. In the absence of an extensive recycle stream, such packed bed reactors are typically modeled as "plug flow". The other most common continuous reactor is a stirred tank, which is assumed to be...
10.4K
Liquid Phase Isoelectric Focusing: An Electrophoresis Technique to Separate Bioactive Molecules from Crude Gymnema sylvestre Extract03:11

Liquid Phase Isoelectric Focusing: An Electrophoresis Technique to Separate Bioactive Molecules from Crude Gymnema sylvestre Extract

This video describes a technique to separate complex molecules from plant extracts using liquid phase isoelectric focusing. This technique helps purify complex biomolecules in their native state which can be used for various downstream...
1.5K
A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

The liquid biopsy has revolutionized our approach to oncology translational studies, with sample collection, quality, and storage being crucial steps for its successful clinical application. Here we describe a standardized and validated protocol for downstream circulating-free DNA applications that can be applied in most translational research...
4.6K