Related Experiment Video
Updated: Jan 19, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Fast structured illumination microscopy with reflectance disturbance resistibility and improved accuracy
This study introduces an improved fast structured illumination microscopy (FSIM) technique to accurately measure surface profiles, even with varying reflectivity. The enhanced method overcomes limitations of traditional FSIM for heterogeneous materials.
Area of Science:
- Optical microscopy
- Surface metrology
- Nanotechnology
Background:
- Conventional fast structured illumination microscopy (FSIM) struggles with non-uniform surface reflectivity, limiting its use for heterogeneous materials.
- Environmental fluctuations and material properties can cause reflectivity variations, affecting profile measurement accuracy.
- Existing methods lack robustness for samples with surface heterogeneity.
Purpose of the Study:
- To develop an improved FSIM (IFSIM) configuration for enhanced axial accuracy and suppressed reflectivity disturbances.
- To enable reliable surface profiling of samples with inhomogeneous reflectivity.
- To offer selective control over dynamic range and axial accuracy.
Main Methods:
- Utilized a digital micro-mirror device (DMD) to project sinusoidal patterns onto the sample.
- Employed two charge-coupled devices (CCDs) placed at different axial positions (in, behind, or before the focal plane).
- Developed a new response function by dividing the sum and subtraction of axial modulation response curves (AMRs) from the two CCDs.
Main Results:
- The proposed IFSIM effectively suppresses the influence of inhomogeneous reflectivity.
- Achieved enhanced axial accuracy and a wide dynamic range by controlling detector defocusing.
- Theoretical analysis and experimental results confirmed the method's feasibility.
Conclusions:
- The improved FSIM (IFSIM) offers a robust solution for accurate surface profiling of heterogeneous materials.
- This technique overcomes the limitations of conventional FSIM in the presence of reflectivity variations.
- IFSIM provides a versatile platform for advanced surface metrology applications.
More Related Videos
12:44Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
Published on: September 29, 2014
14:11Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
Published on: May 4, 2014
Related Concept Videos
11:15A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
12:44Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
Improving Translational Accuracy
Improving Translational Accuracy
14:11Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
07:53Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy