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Related Concept Videos

Label-Retention Expansion Microscopy (LR-ExM) Enables Super-Resolution Imaging and High-Efficiency Labeling07:44

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Related Experiment Video

Updated: Jan 19, 2026

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
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Super-condenser enables labelfree nanoscopy.

Florian Ströhl, Ida S Opstad, Jean-Claude Tinguely

    Optics Express
    |September 13, 2019
    PubMed
    Summary
    This summary is machine-generated.

    We developed a novel labelfree nanoscopy method using waveguide microscopy and Fourier ptychography for high-resolution imaging. This technique offers an alternative to traditional methods, though it may produce image artifacts.

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    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Nanotechnology

    Background:

    • Labelfree nanoscopy achieves ~100 nm resolution using visible light.
    • Existing methods may have limitations in resolution or require labels.

    Purpose of the Study:

    • To present a novel labelfree nanoscopy method combining coherent imaging and waveguide microscopy.
    • To achieve super-resolution imaging over larger areas using Fourier ptychography.

    Main Methods:

    • Utilized silicon nitride (Si3N4) waveguides for maximally inclined coherent darkfield illumination.
    • Employed adiabatically tapered waveguides for coherent plane wave illumination.
    • Manipulated illumination wave vectors via waveguide input structures and wavelength changes for Fourier ptychography phase retrieval.

    Main Results:

    • Demonstrated a labelfree nanoscopy technique with Fourier ptychography over 400 µm² imaging areas.
    • Identified image artifacts in Fourier ptychography reconstructions.
    • Showcased rotating coherent scattering microscopy (ROCS) as a more robust, albeit lower-resolution, alternative.

    Conclusions:

    • The developed method shows promise for labelfree super-resolution imaging.
    • Fourier ptychography reconstructions require careful artifact management.
    • ROCS provides a robust alternative for specific applications.