Related Concept Videos

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis07:58

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

In this protocol, the pancreatic islets are reconstructed and analyzed using computational algorithms implemented in a dedicated multiplatform...
1.9K
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells07:21

Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells

Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from...
4.8K
Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells08:03

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is...
8.8K
Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices05:51

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

This study presents the application of live pancreatic tissue slices to the study of islet physiology and islet-immune cell interactions.
4.6K
A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Critical challenges for the diabetes research field are to understand the molecular mechanisms that regulate islet β-cell replication and to develop methods for stimulating β-cell regeneration. Herein a high-content screening method to identify and assess the β-cell replication-promoting activity of small molecules is...
11.6K
Pancreatic Islet Embedding for Paraffin Sections09:09

Pancreatic Islet Embedding for Paraffin Sections

Ex vivo pancreatic islet studies are important for diabetes research. Existing techniques to study cultured islets in their native 3-dimensional architecture are time consuming, inefficient, and infrequently used. This work describes a new, simple, and efficient method for generating high-quality paraffin sections of whole cultured...
13.5K