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Related Concept Videos

Axonal Regeneration in the Sciatic Nerve of a Mouse Following a Crush Injury02:40

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Source: Li, Q., et al. Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion. J. Vis. Exp. (2018)This video demonstrates the process of axonal regeneration in a mouse sciatic nerve following a crush injury. The isolated nerve is fixed and prepared for imaging, with fluorescent proteins expressed in neurons to visualize axon growth. Using an epifluorescence microscope, axon regeneration is traced from the injury site to assess the extent of...
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Related Experiment Video

Updated: Jan 19, 2026

Axonal Regeneration in the Sciatic Nerve of a Mouse Following a Crush Injury
02:40

Axonal Regeneration in the Sciatic Nerve of a Mouse Following a Crush Injury

Published on: August 29, 2025

337

Sciatic nerve regeneration by using collagen type I hydrogel containing naringin.

Hadi Samadian1, Ahmad Vaez2, Arian Ehterami3

  • 1Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Journal of Materials Science. Materials in Medicine
|September 13, 2019
PubMed
Summary

This study developed a collagen hydrogel with naringin for peripheral nerve repair. The naringin-loaded hydrogel significantly improved nerve function and regeneration in animal models.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Peripheral nerve injuries pose significant challenges in treatment, often requiring advanced biomaterials for effective regeneration.
  • Collagen hydrogels offer a promising biocompatible scaffold, but their efficacy can be enhanced with bioactive compounds.

Purpose of the Study:

  • To fabricate and characterize a novel collagen hydrogel incorporating naringin for peripheral nerve damage treatment.
  • To evaluate the in vitro and in vivo efficacy of the naringin-loaded collagen hydrogel in promoting nerve repair.

Main Methods:

  • Collagen hydrogels were synthesized using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) cross-linking and loaded with naringin.
  • Hydrogels were characterized for microstructure, swelling, biodegradation, and cyto/hemocompatibility.
  • In vitro studies assessed Schwann cell proliferation, while in vivo studies utilized a rat sciatic nerve crush injury model.

Main Results:

  • The collagen/naringin hydrogel exhibited a porous microstructure (90 µm pores) and approximately 70% degradation over 4 weeks.
  • In vitro, Schwann cell proliferation was significantly enhanced on the collagen/naringin hydrogel compared to controls.
  • In vivo, the hydrogel significantly improved sciatic nerve functional recovery, with histological analysis showing well-arranged nerve fibers and intact myelin.

Conclusions:

  • The developed collagen/naringin hydrogel demonstrates excellent biocompatibility and promotes peripheral nerve regeneration.
  • This naringin-loaded collagen hydrogel represents a promising advanced alternative for treating peripheral nerve injuries.