Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses04:07

Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses

1.3K
This video describes a detailed protocol for producing genetically modified primary human keratinocytes recombinant retrovirus. This method can be used to manipulate gene expression in...
1.3K
Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification11:44

Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

4.9K
The electroporation of primate cerebral organoids provides a precise and efficient approach to introduce transient genetic modification(s) into different progenitor types and neurons in a model system close to primate (patho)physiological neocortex development. This allows the study of neurodevelopmental and evolutionary processes and can also be applied for disease...
4.9K
Fluorimetric Techniques for the Assessment of Sperm Membranes08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

14.1K
Here, we present methodologies to evaluate spermatozoan membrane integrity, a cellular feature associated with sperm fertilization competence. We describe three techniques for the fluorimetric assessment of sperm membranes: simultaneous staining with specific fluorescent probes, fluorescence microscopy, and advanced sperm-dedicated flow cytometry. Examples of combining the methodologies are also...
14.1K
Agrobacterium-Mediated Genetic Transformation: A Method to Genetically Transform the Rice Genome via Genetically Engineered Agrobacterium tumefaciens05:01

Agrobacterium-Mediated Genetic Transformation: A Method to Genetically Transform the Rice Genome via Genetically Engineered Agrobacterium tumefaciens

5.1K
In this video, we demonstrate genetic transformation of the rice genome using an Agrobacterium tumefaciens binary vector. This bacterium facilitates the transfer of a foreign DNA plasmid using Agrobacterium virulence...
5.1K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.3K
Stereotaxic Injection of Viruses for Stable Genetic Modification in a Mouse Model04:12

Stereotaxic Injection of Viruses for Stable Genetic Modification in a Mouse Model

282
Source: Porlan, E., et al. Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects. J. Vis. Exp. (2016)This video demonstrates stable genetic modification of neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ) through stereotaxic injection of a virus-carrying therapeutic gene in a mouse model. The virus enters NSCs, undergoes reverse transcription, integrates into the genome, and...
282

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Long-term neurological outcome after hematopoietic stem cell transplant in juvenile Krabbe disease.

Journal of neurology·2026
Same author

Beyond <i>Aspergillus fumigatus</i>: The Clinical Burden of <i>Aspergillus flavus</i> and <i>Aspergillus niger</i> in Chronic Pulmonary Diseases.

Pathogens (Basel, Switzerland)·2026
Same author

Aroma diversification and formation of bioactive tyrosol and tryptophol acetates by the yeast <i>Hanseniaspora vineae</i> during cider fermentation.

Frontiers in nutrition·2026
Same author

Simulated Mars Gravity Impairs Intestinal Epithelial Barrier Integrity via Selective Modulation of Tight Junction Components.

Biomolecules·2026
Same author

A FHIR Consent Profile for European Research Biobanks.

Studies in health technology and informatics·2026
Same author

Improving the compatibility of INFOGEST digesta with intestinal epithelial models through post-digestion storage conditions.

Scientific reports·2026

Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification
11:44

Author Spotlight: Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

Published on: March 24, 2023

4.9K

Sperm-Mediated Genetic Modifications.

Marialuisa Lavitrano1, Laura Farina2, Maria Grazia Cerrito2

  • 1School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy. marialuisa.lavitrano@unimib.it.

Methods in Molecular Biology (Clifton, N.J.)
|September 13, 2019
PubMed
Summary
This summary is machine-generated.

Sperm-mediated gene transfer enables creating genetically modified animals by utilizing sperm's natural ability to carry foreign DNA to the egg during fertilization for research applications.

Keywords:
MouseSMGTSpermatozoaTransgenesisTransgenic animals

More Related Videos

Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses
04:07

Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses

Published on: April 30, 2023

1.3K
Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.1K

Related Experiment Videos

Last Updated: Jan 19, 2026

Author Spotlight: Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification
11:44

Author Spotlight: Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

Published on: March 24, 2023

4.9K
Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses
04:07

Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses

Published on: April 30, 2023

1.3K
Fluorimetric Techniques for the Assessment of Sperm Membranes
08:58

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

14.1K

Area of Science:

  • Animal genetics and reproductive biology.
  • Biotechnology and genetic engineering.
  • Transgenic animal models for research.

Background:

  • Controlled genome modification is crucial for biomedical and veterinary research.
  • Various transgenic techniques exist for animal genetic manipulation.
  • Sperm-mediated gene transfer offers a unique approach to genetic modification.

Purpose of the Study:

  • To describe the sperm-mediated gene transfer method.
  • To highlight its application in producing genetically modified animals.
  • To emphasize its utility in research settings.

Main Methods:

  • Utilizing the inherent capacity of sperm cells to bind and internalize exogenous DNA.
  • Employing sperm as natural vectors for DNA delivery.
  • Facilitating gene transfer during the fertilization process.

Main Results:

  • Successful production of genetically modified animals via sperm-mediated gene transfer.
  • Demonstration of sperm's ability to transport and deliver foreign DNA to the oocyte.
  • Validation of the technique for creating transgenic animal models.

Conclusions:

  • Sperm-mediated gene transfer is an effective method for generating genetically modified animals.
  • This technique leverages natural biological processes for efficient gene transfer.
  • It provides a valuable tool for advancing biomedical and veterinary research through genetic modification.