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Silencing long noncoding RNA OGFRP1 inhibits the proliferation and migration of cervical carcinoma cells
Kun Zou1, Haifeng Yu1, Xuehua Chen1
1Jinan Central Hospital, Jinan, Shandong, China.
Abstract:
Cervical cancer is still a serious threat to women's health and life safety worldwide, and new treatment strategies are urgently needed. Accumulating evidences also imply that long non-coding RNAs (lncRNAs) are involved in a wide range of cellular processes, such as cell proliferation, apoptosis, and cell cycle. We found that the expression of lncOGFRP1 in cervical cancer tissues was significantly higher than that in normal cervical tissues (P < .05). Further, CCK8 detection found when lncOGFRP1 was silenced, the proliferation of cells was inhibited. After depleting lncOGFRP1, the proportion of apoptosis cells in C33A (3.71 ± 0.38% VS 11.98 ± 1.26%, P < .05) and SiHa (0.69 ± 0.06% VS 11.06 ± 1.03%, P < .05) cells increased significantly, and cell cycle was arrested in S phase. On the other hand, migration detection found the migration of cells also was hindered when lncOGFRP1 level was reduced. And the depletion of lncOGFRP1 inhibited the expression of β-catenin, Vimentin, N-cadherin, and SNAIL and promoted the expression of E-cadherin. In summary, we first discovered the high expression of lncOGFRP1 in cervical cancer and revealed that silencing lncOGFRP1 inhibits the proliferation and migration of cervical carcinoma cells. SIGNIFICANCE OF THE STUDY: We first discovered the high expression of lncOGFRP1 in cervical cancer and revealed that silencing lncOGFRP1 inhibits the proliferation and migration of cervical carcinoma cells. These results help to better understand the pathogenesis and development of cervical cancer and provide insight to develop better diagnosis and treatment strategies.
Insights
High expression of long non-coding RNA OGFRP1 (lncOGFRP1) is linked to cervical cancer. Silencing lncOGFRP1 inhibits cancer cell proliferation and migration, offering potential new treatment strategies.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Cervical cancer remains a significant global health concern, necessitating novel therapeutic approaches.
- Long non-coding RNAs (lncRNAs) play crucial roles in cellular processes relevant to cancer, including proliferation and apoptosis.
Purpose of the Study:
- To investigate the role of lncOGFRP1 in cervical cancer.
- To determine the impact of lncOGFRP1 expression on cervical carcinoma cell behavior.
Main Methods:
- Quantitative analysis of lncOGFRP1 expression in cervical cancer tissues versus normal tissues.
- Cell proliferation assays (CCK8) following lncOGFRP1 silencing.
- Apoptosis assays and cell cycle analysis.
- Cell migration assays.
- Western blot analysis of key proteins involved in epithelial-mesenchymal transition (EMT).
Main Results:
- lncOGFRP1 expression was significantly elevated in cervical cancer tissues.
- Silencing lncOGFRP1 inhibited cervical carcinoma cell proliferation and induced apoptosis.
- Depletion of lncOGFRP1 led to S-phase cell cycle arrest and reduced cell migration.
- lncOGFRP1 silencing downregulated EMT markers (β-catenin, Vimentin, N-cadherin, SNAIL) and upregulated E-cadherin.
Conclusions:
- This study identifies lncOGFRP1 as a novel oncogene in cervical cancer with high expression.
- Silencing lncOGFRP1 effectively inhibits cervical cancer cell proliferation and migration.
- lncOGFRP1 may serve as a potential diagnostic marker and therapeutic target for cervical cancer.
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