Related Concept Videos

A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds03:37

A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds

This video showcases the deferred growth inhibition assay, used to investigate how antimicrobial compounds produced by one type of bacteria affect another competing bacterial...
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Evaluating the Effect of Beneficial Bacteria on Gut Microbial Translocation in Heat-Stressed Rats02:47

Evaluating the Effect of Beneficial Bacteria on Gut Microbial Translocation in Heat-Stressed Rats

Source: Sorokulova, I., et al. Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain. J. Vis. Exp. (2016)This video demonstrates rats pre-treated with beneficial bacteria or phosphate-buffered saline (PBS) and exposed to heat stress to evaluate gut barrier integrity. Fewer gut-derived bacterial colonies from the liver of treated rats, in contrast to numerous colonies in control rats, indicate an intact epithelial barrier and minimal bacterial...
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Quantification of Colony-Forming Units of Gut-Associated Bacteria in Drosophila Flies06:37

Quantification of Colony-Forming Units of Gut-Associated Bacteria in Drosophila Flies

Source: Dodge, R., et al. Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome. J. Vis. Exp. (2023)This video demonstrates the quantification of colony-forming units (CFUs) of gut-associated bacteria in Drosophila flies using a serial dilution and plating approach. Homogenized fly samples containing gut bacteria are serially diluted, plated on agar, and incubated to allow colony growth. Images of the plates are then analyzed using software to align,...
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Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition07:42

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

The deferred growth inhibition assay can be used to assess the competition effect by one bacterial isolate on another. Inhibition is quantified by measuring the zone of clearing around the inhibitor-producing isolate, or qualitatively assessed by determining the visible extent of...
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Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose12:11

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

The active bacterial community associated with the gut of Spodoptera littoralis, was determined by stable-isotope-probing (SIP) coupled to pyrosequencing. Using this methodology, identification of the metabolically active bacteria species within the community was done with high resolution and...
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An In Vitro Technique to Study Antimicrobial Treatments on Bacterial Aggregates04:12

An In Vitro Technique to Study Antimicrobial Treatments on Bacterial Aggregates

This video demonstrates an in vitro technique to study the impact of antimicrobial treatment on Pseudomonas aeruginosa (Pa) aggregates. Fluorescently-labeled Pa cells are grown as aggregates, mimicking the growth pattern during infection. Upon challenging the aggregates with a sublethal concentration of the antibiotic colistin, the real-time assessment of the bacteria-antibiotic interaction is performed via fluorescence...
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