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Related Experiment Video

Updated: Jan 19, 2026

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A multilocus sequence typing scheme for Mycobacterium abscessus complex (MAB-multilocus sequence typing) using

Michelle Wuzinski1, Aneta K Bak2, Aaron Petkau3

  • 1National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Public Health Agency of Canada; Department of Microbiology, University of Manitoba, Manitoba, Canada.

International Journal of Mycobacteriology
|September 13, 2019
PubMed
Summary
This summary is machine-generated.

A new multilocus sequence typing scheme, MAB-MLST, was developed for Mycobacterium abscessus, improving outbreak investigation and diagnostics. This scheme enhances the ability to track transmission and genomic evolution of this significant pathogen.

Keywords:
MAB-multilocus sequence typingMycobacterium abscessusNontuberculous mycobacteriamultilocus sequence typingrapid growing mycobacteria

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Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Mycobacterium abscessus is a rapidly growing nontuberculous mycobacteria (NTM) causing difficult-to-treat human infections.
  • NTM outbreaks require complex investigations and treatment strategies.
  • Limited typing schemes exist for NTMs like M. abscessus, hindering transmission tracking and genomic evolution studies.

Purpose of the Study:

  • To develop an extended multilocus sequence typing (MLST) scheme for M. abscessus using whole-genome data.
  • To enhance the discriminatory power for subtyping M. abscessus complex isolates.
  • To provide a tool for improved outbreak investigations and routine diagnostics.

Main Methods:

  • Extended the existing PubMLST scheme by incorporating data from 15 genetic regions within the M. abscessus genome.
  • Utilized 168 whole genomes and 11 gene sequences to build the MAB-MLST scheme.
  • Added eight novel genes to the existing seven PubMLST genes, increasing the number of alleles and sequence types.

Main Results:

  • The MAB-MLST scheme identified 85 unique sequence types (STs) among the isolates.
  • Expanded gene set includes argH, cya, gnd, murC, pta, purH, rpoB, hsp 65, erm(41), arr, rrs, rrl, gyrA, gyrB, and recA.
  • Subtyped 90 additional isolates not previously genotyped by PubMLST, with results comparable to whole-genome sequencing (WGS).

Conclusions:

  • MAB-MLST enables subspecies-level identification of M. abscessus complex using three genes.
  • Antimicrobial resistance susceptibility prediction is possible with seven genes.
  • A Galaxy-based tool simplifies WGS data analysis, aiding outbreak deciphering and routine diagnostics with high discriminatory power.