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Related Experiment Videos

Semen preservation in Macaca fascicularis.

J P Mahone, W R Dukelow

    Laboratory Animal Science
    |October 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Optimizing semen cryopreservation for Macaca fascicularis involves specific extender conditions. Key factors for maintaining sperm motility include pH 7.2-8.0, 7-10% glycerol, and a 1-minute equilibration time.

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    Area of Science:

    • Veterinary Science
    • Reproductive Biology
    • Cryopreservation Technology

    Background:

    • Semen cryopreservation is crucial for the genetic management of non-human primates.
    • Macaca fascicularis (crab-eating macaques) are important models in biomedical research.
    • Optimizing semen extenders is essential for successful sperm cryopreservation.

    Purpose of the Study:

    • To determine the optimal extender composition and equilibration time for cryopreservation of Macaca fascicularis semen.
    • To evaluate the impact of egg yolk concentration, pH, glycerol concentration, and equilibration time on sperm motility.

    Main Methods:

    • Semen was collected from adult male Macaca fascicularis via electro-ejaculation.
    • Sperm motility was assessed after cryopreservation using varying extender egg yolk concentrations (10-40%), pH levels (5.8-8.7), glycerol concentrations (7-10%), and equilibration times (1, 25, 45 minutes).

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    Main Results:

    • No significant difference in sperm motility was observed across egg yolk concentrations from 10% to 40%.
    • Progressive motility was significantly higher at pH 7.2 and 8.0 compared to lower or higher pH values (p < 0.05).
    • Optimal progressive motility was achieved with 7% and 10% glycerol concentrations.
    • A 1-minute equilibration time resulted in superior sperm motility post-cryopreservation compared to 25 or 45 minutes.

    Conclusions:

    • Semen extender pH and glycerol concentration significantly impact Macaca fascicularis sperm motility after cryopreservation.
    • A short equilibration time of 1 minute is optimal for preserving sperm motility.
    • These findings provide critical data for improving cryopreservation protocols for Macaca fascicularis semen.