Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An electronic method for counting human T and B cell rosettes.

T N Denny, K M Kinsley, J W Benson

    Vox Sanguinis
    |January 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Marshall Hall's Ready Method in Asphyxia of the Newly-Born, Etc.; Hysteria from Chloroform.

    The Chicago medical journal·2023
    Same author

    Scarlet Fever.

    Atlanta medical and surgical journal·2022
    Same author

    Transmission of bovine leukemia virus: Prevalence of antibodies in precolostral calves.

    Preventive veterinary medicine·2020
    Same author

    The Immunology Quality Assessment Proficiency Testing Program for CD3⁺4⁺ and CD3⁺8⁺ lymphocyte subsets: a ten year review via longitudinal mixed effects modeling.

    Journal of immunological methods·2014
    Same author

    The dynamics of bone marrow stromal cells in the proliferation of multipotent hematopoietic progenitors by substance P: an understanding of the effects of a neurotransmitter on the differentiating hematopoietic stem cell.

    Journal of neuroimmunology·2001
    Same author

    Relationship between oxidative burst activity and CD11b expression in neutrophils and monocytes from healthy individuals: effects of race and gender.

    Cytometry·2001
    Same journal

    Barriers and enablers to non-remunerated plasma donation: A meta-synthesis of the qualitative literature using the theoretical domains framework.

    Vox sanguinis·2026
    Same journal

    Haemolytic disease of the foetus and newborn due to anti-M: A systematic review.

    Vox sanguinis·2026
    Same journal

    In vitro evaluation of apheresis platelet and plasma products collected and stored in non-DEHP disposable sets.

    Vox sanguinis·2026
    Same journal

    Survey of national and regional rare donor programmes regarding Immunoglobulin A deficiency.

    Vox sanguinis·2026
    Same journal

    Fibrinogen recovery in cryoprecipitate prepared from thawed plasma stored for 5 days post-thaw.

    Vox sanguinis·2026
    Same journal

    Abstracts of the 39th International Congress of the ISBT, Kuala Lumpur, Malaysia, 20-24 June 2026.

    Vox sanguinis·2026
    See all related articles

    An electronic particle counter offers a rapid and convenient method for enumerating human lymphocyte T cell and B cell rosettes. This semi-automated technique shows significant agreement with traditional hemocytometer methods.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biotechnology

    Background:

    • Accurate enumeration of T cell and B cell rosettes is crucial for immunological studies.
    • Traditional hemocytometer methods can be time-consuming and prone to manual error.

    Purpose of the Study:

    • To adapt and validate an electronic particle-counting method for enumerating human lymphocyte T cell and B cell rosettes.
    • To compare the performance of the electronic particle counter with the conventional hemocytometer method.

    Main Methods:

    • A blind study design was employed, comparing the electronic particle-counting method with the hemocytometer method.
    • Seventeen T cell and seven B cell rosette enumerations were performed in parallel.
    • Statistical analysis, including correlation coefficients, was used to assess agreement between methods.

    Related Experiment Videos

    Main Results:

    • Significant agreement was found between the electronic particle counter and hemocytometer methods for T cell rosettes (p < 0.001).
    • Significant agreement was also observed for B cell rosettes (p < 0.002).
    • The electronic particle counter yielded mean values of 80.6% +/-11.3 for T cells and 22.5% +/-9.7 for B cells.

    Conclusions:

    • The electronic particle counter is a reliable and effective tool for enumerating T cell and B cell rosettes.
    • This semi-automated method offers a rapid and convenient alternative to conventional techniques.
    • The findings support the utility of electronic particle counting in immunological research.