Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil

Strain KUDC5002^T was isolated from soil sampled on the Dokdo Islands, Republic of Korea. This bacterial strain was Gram stain-positive, non-motile, rod-shaped, capable of growing at 25-37°C and pH 5.0-12.0, and showed optimal growth at 30 °C and pH 7.0-8.0. Strain KUDC5002^T could be grown in tryptic soy broth containing less than 7.0 % NaCl (w/v). The cell width ranged from 0.5 to 0.6 µm and length ranged from 0.8 to 1.0 µm. Strain KUDC5002^T was catalase- and oxidase-positive. Its genomic G+C content was 72.2 mol%. Its major fatty acids were C_18 : 1 ω9c (17.3 %), iso-C_16 : 0 (16.0 %) and iso-C_17 : 0 (11.4 %). Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain KUDC5002^T belongs to the genus Nocardioides and is most closely related to strain Nocardioides humi DCY24^T (97.0 %). Based on its phenotypic, phylogenetic, genetic and chemotaxonomic features, strain KUDC5002^T should be considered a novel species in the genus Nocardioides, for which we have proposed the name Nocardioides sambongensis sp. nov. The type strain is KUDC5002^T (=KCTC 39855^T=DSM 106604^T).