Flavobacterium cellulosilyticum sp. nov., a novel psychrophilic bacterium isolated from Arctic soil

A novel psychrophilic, light-yellow-coloured, aerobic, Gram-stain-negative, rod-shaped, non-motile bacterium, designated strain AR-3-4^T was isolated from a sample of Arctic soil. Strain AR-3-4^T grew at 0-25 °C, pH 6.0-9.0 and 0-1.0 % (w/v) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain AR-3-4^T belonged to the genus Flavobacterium, with nearest phylogenetic neighbour being Flavobacterium fluvii H7^T (97.5 % sequence similarity). The strain comprised phosphatidylethanolamine as the main polar lipid, MK-6 as predominant respiratory quinone, and summed feature 3 (C_16 : 1ω7c and/or C_16 : 1ω6c), anteiso-C_15 : 0 and iso-C_15 : 0 as the major fatty acids. The average nucleotide identity and in silico DNA-DNA hybridization values between strain AR-3-4^T and closest members were below the threshold values of 95 % and 70 %, respectively. The DNA G+C content was 33.0 mol%. Based on the polyphasic taxonomic data, the novel species Flavobacterium cellulosilyticum sp. nov. is proposed with the type strain AR-3-4^T (=KEMB 9005-740^T=KACC 21171^T=NBRC 113787^T).