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Related Concept Videos

DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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DT40, a model vertebrate genetic system, provides a powerful tool to analyze protein function. Here we describe a simple method that allows qualitative analysis of parameters that influence DNA synthesis during the S-phase in DT40 cells at the single molecule...
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Chromosome Replication02:31

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Replication in Eukaryotes02:31

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Related Experiment Video

Updated: Jan 19, 2026

DNA Replication: Semiconservative, 5'-3' DNA Synthesis
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DNA Replication: Semiconservative, 5'-3' DNA Synthesis

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Origins of DNA replication.

Babatunde Ekundayo1, Franziska Bleichert1

  • 1Quantitative Biology, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Plos Genetics
|September 13, 2019
PubMed
Summary

DNA replication ensures genetic material passes to daughter cells. This study explores how DNA replication origins are organized and recognized across different life forms, highlighting conserved and divergent strategies.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA replication is essential for cell division and heredity.
  • DNA synthesis begins at replication origins and proceeds bidirectionally.
  • Organisms exhibit diverse mechanisms for controlling replication initiation.

Purpose of the Study:

  • To compare and contrast replication origin organization and recognition.
  • To identify commonalities and differences across the three domains of life.

Main Methods:

  • Comparative analysis of DNA replication initiation mechanisms.
  • Review of existing literature on replication origins.

Main Results:

  • Replication origin organization and recognition vary significantly among archaea, bacteria, and eukaryotes.

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  • Despite differences, fundamental principles of replication initiation are conserved.

    • Conclusions:

    • Understanding these diverse strategies provides insight into genome stability and evolution.
    • Further research can elucidate the specific molecular players involved in replication control.