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Related Experiment Video

Updated: Jan 19, 2026

Production of Human CRISPR-Engineered CAR-T Cells

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

CRISPR technologies for stem cell engineering and regenerative medicine.

Mu-Nung Hsu¹, Yu-Han Chang², Vu Anh Truong¹

¹Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.

Biotechnology Advances

|September 13, 2019

Summary

CRISPR technology, including CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa), offers precise gene control. These tools are advancing stem cell engineering and regenerative medicine for various diseases.

Keywords:

CRISPR CRISPRa CRISPRi Genome editing Regenerative medicine Stem cell

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Published on: March 10, 2023

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Last Updated: Jan 19, 2026

Production of Human CRISPR-Engineered CAR-T Cells

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Published on: March 15, 2021

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

The CRISPR/Cas9 system is a powerful tool for genome editing.
Catalytically deactivated Cas9 (dCas9) enables targeted gene regulation without cutting DNA.
dCas9 can be fused with transcriptional repressors (CRISPRi) or activators (CRISPRa).

Purpose of the Study:

To review the principles and recent advances in CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) technologies.
To discuss the applications of these CRISPR tools in stem cell engineering and regenerative medicine.
To explore challenges and future perspectives for translating CRISPR technologies into clinical applications.

Main Methods:

Review of CRISPR/Cas9 system, dCas9 modifications, and CRISPRi/CRISPRa mechanisms.
Analysis of delivery vectors for CRISPR components.
Compilation and synthesis of recent research on CRISPR applications in regenerative medicine.

Main Results:

CRISPRi and CRISPRa provide precise control over gene expression for various applications.
These technologies have shown promise in in vitro stem cell fate manipulation.
Successful in vivo applications include preventing retinal and muscular degeneration, promoting neural and bone regeneration, and treating blood, skin, and liver diseases.

Conclusions:

CRISPRi and CRISPRa are versatile tools for gene regulation with significant potential in regenerative medicine.
Further development and overcoming delivery challenges are crucial for clinical translation.
CRISPR technologies hold promise for treating a wide range of degenerative diseases and injuries.