Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

584
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
584
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release06:02

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

4.3K
The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and...
4.3K
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents04:19

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

22.2K
Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is...
22.2K
Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography10:40

Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography

1.5K
We present a step-by-step protocol for high-resolution, label-free, and three-dimensional imaging of organoids using low-coherence holotomography. This protocol details organoid culture preparation, imaging acquisition, and computational image analysis, enabling real-time visualization of structural dynamics and drug responses in living...
1.5K
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning08:58

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

13.0K
We describe a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and a machine learning algorithm. Measurements of 3D refractive index tomograms of lymphocytes present 3D morphological and biochemical information for individual cells, which is then analyzed with a machine-learning algorithm for identification of cell...
13.0K
Metabolic Labeling08:28

Metabolic Labeling

13.1K
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
13.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mineral-biofilm interaction controls trophic transfer of PFAS along a biofilm-snail food chain.

Aquatic toxicology (Amsterdam, Netherlands)·2026
Same author

The patterns of microbial community distribution and co-occurrence in water columns and sediments of Haima cold seep.

Microbiology spectrum·2026
Same author

LIFT+: Lightweight Fine-Tuning for Long-Tail Learning.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Construction of high-density genetic map and QTL mapping of flowering traits in Perilla frutescens.

BMC plant biology·2026
Same author

Topical framework nucleic acid transdermal delivery system for reprogramming cutaneous dendritic cells to maintain graft immune homeostasis.

Bioactive materials·2026
Same author

Surface reconstruction-driven WCuNiMo alloy for efficient ammonia oxidation and hydrogen evolution.

Nanoscale·2026
Same journal

Intervention Feasible Region and Driver Risk Capacity Aware Human-Machine Collaborative Safe Trajectory Planning.

IEEE transactions on neural networks and learning systems·2026
Same journal

A Unified Differential Denoising Learning Framework With a Pre-Trained Model and Fuzzy Graph Networks for Drug-Drug Interaction Prediction.

IEEE transactions on neural networks and learning systems·2026
Same journal

Self-Supervised Continuous Dynamic Graph Representation Learning via Hawkes Processes.

IEEE transactions on neural networks and learning systems·2026
Same journal

cPU: Consistent Risk Estimator for Positive-Unlabeled Learning.

IEEE transactions on neural networks and learning systems·2026
Same journal

Tuning-Free Latent Diffusion Models for Ultrahigh-Resolution Image Editing.

IEEE transactions on neural networks and learning systems·2026
Same journal

Hidden Data Recovery and Forecasting via Next-Generation Reservoir Computing With Multiscale Delay Selection.

IEEE transactions on neural networks and learning systems·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

584

Does Tail Label Help for Large-Scale Multi-Label Learning?

Tong Wei, Yu-Feng Li

    IEEE Transactions on Neural Networks and Learning Systems
    |September 13, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Large-scale multi-label learning (LMLL) research reveals tail labels have minimal impact on performance metrics. New low-complexity LMLL methods reduce prediction time and model size by focusing on influential labels.

    More Related Videos

    Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
    06:02

    Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

    Published on: June 12, 2021

    4.3K
    A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
    04:19

    A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

    Published on: April 19, 2019

    22.2K

    Related Experiment Videos

    Last Updated: Jan 19, 2026

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
    09:19

    Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

    Published on: August 29, 2025

    584
    Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
    06:02

    Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

    Published on: June 12, 2021

    4.3K
    A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
    04:19

    A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

    Published on: April 19, 2019

    22.2K

    Area of Science:

    • Machine Learning
    • Data Science
    • Artificial Intelligence

    Background:

    • Large-scale multi-label learning (LMLL) involves annotating unseen data with relevant labels from a vast set of candidates.
    • Labels in LMLL often follow a long-tail distribution, with numerous infrequent 'tail' labels.
    • Previous research assumed tail labels improve performance, but their specific impact remained unquantified.

    Purpose of the Study:

    • To quantify the impact of tail labels on LMLL performance metrics.
    • To develop efficient LMLL methods by identifying and leveraging less influential labels.
    • To reduce computational complexity, prediction time, and model size in LMLL.

    Main Methods:

    • Analyzed the impact of label frequency and weight on LMLL evaluation metrics like Propensity Score Precision (PSP)@k and Propensity Score nDCG (PSnDCG)@k.
    • Proposed low-complexity LMLL methods that strategically restrain less performance-influential labels.
    • Developed a technique to preserve dominant model parameters for these less influential labels to mitigate performance loss.

    Main Results:

    • Demonstrated that label impact on PSP@k and PSnDCG@k is proportional to the product of label weights and frequencies.
    • Showcased that tail labels, especially with equal weights, have significantly less impact due to lower frequencies.
    • Empirically validated that the proposed methods substantially reduce prediction time and model size without significant performance degradation.

    Conclusions:

    • The impact of labels in LMLL is quantifiable and directly related to their frequency and weight.
    • Low-complexity LMLL methods can be developed by focusing on high-impact labels, leading to efficiency gains.
    • The proposed approach effectively balances model efficiency with predictive performance in large-scale multi-label learning scenarios.