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Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response06:31

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

Minimal erythema dose (MED) testing is used to establish dosage schedules for ultraviolet radiation phototherapy. It can assess individual variation in inflammatory response but lacks methodology for achieving reproducible results. Here, we present a precision implementation of MED and demonstrate its ability to capture individual variation in inflammatory...
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Laminectomy and Spinal Cord Window Implantation in the Mouse06:59

Laminectomy and Spinal Cord Window Implantation in the Mouse

This protocol describes implantation of a glass window onto the spinal cord of a mouse to facilitate visualization by intravital...
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Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis09:20

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon...
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Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice06:33

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Presented here is a protocol for the implantation of a chronic cranial window for the longitudinal imaging of brain cells in awake, head-restrained...
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Spinal Cord Window Implantation in a Mouse for In Vivo Imaging03:14

Spinal Cord Window Implantation in a Mouse for In Vivo Imaging

Source: Pietruczyk, E. A., et al. Laminectomy and Spinal Cord Window Implantation in the Mouse. J. Vis. Exp. (2019)This video demonstrates the implantation of a spinal cord window in an anesthetized mouse for in vivo imaging. The protocol stabilizes the exposed spinal cord using a borosilicate cover glass and a 3D-printed backplate. This method enables high-resolution two-photon microscopy to study blood-brain barrier dynamics and neurovascular...
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Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

This protocol describes the fabrication of an implantable integrated imaging window using 3D laser printing. The window consists of a system of microlenses coupled with micro-scaffolds. The method involves two-photon polymerization (2PP) of the biocompatible photoresist SZ2080 in a continuous sequence, optimizing manufacturing efficiency and alignment between the different...
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