Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry09:20

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

3.8K
MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or...
3.8K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

14.2K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
14.2K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

4.2K
4.2K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.8K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.8K
Regulated Protein Degradation02:58

Regulated Protein Degradation

3.1K
3.1K
MRI and PET in Mouse Models of Myocardial Infarction10:46

MRI and PET in Mouse Models of Myocardial Infarction

12.3K
We describe how to perform MRI and PET imaging of the mouse heart. The protocol is tailored to assess treatment efficacy in models of myocardial infarction and heart...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural and biochemical insights into an anthocyanin-related glutathione transferase from bilberry and its inhibition by quercetin.

International journal of biological macromolecules·2026
Same author

Discovery and engineering of polymerases and ligases for the synthesis of modified nucleic acids.

Current opinion in chemical biology·2026
Same author

Building an oral peptide drug.

Science (New York, N.Y.)·2026
Same author

Biomass-Derived Diformylxylose as a Renewable Solvent for Biocatalysis Applications.

ChemSusChem·2026
Same author

Toward the Chemoenzymatic Synthesis of DNA-Encoded Libraries.

ACS central science·2026
Same author

Of Revolutions and Roadblocks: The Emerging Role of Machine Learning in Biocatalysis.

ACS central science·2025

Related Experiment Video

Updated: Jan 19, 2026

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
09:20

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Published on: April 11, 2022

3.8K

Enzymatic PET Degradation.

Athena Papadopoulou1, Katrin Hecht1, Rebecca Buller2

  • 1Competence Center for Biocatalysis, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, CH-8820 Wädenswil.

Chimia
|September 14, 2019
PubMed
Summary
This summary is machine-generated.

Enzymes can now degrade polyethylene terephthalate (PET) plastic, offering a biological recycling solution. Protein engineering enhances these enzymes, paving the way for a circular plastic economy and sustainable waste management.

More Related Videos

MRI and PET in Mouse Models of Myocardial Infarction
10:46

MRI and PET in Mouse Models of Myocardial Infarction

Published on: December 19, 2013

12.3K
Proteins: From Genes to Degradation
02:11

Proteins: From Genes to Degradation

14.2K

Related Experiment Videos

Last Updated: Jan 19, 2026

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
09:20

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Published on: April 11, 2022

3.8K
MRI and PET in Mouse Models of Myocardial Infarction
10:46

MRI and PET in Mouse Models of Myocardial Infarction

Published on: December 19, 2013

12.3K
Proteins: From Genes to Degradation
02:11

Proteins: From Genes to Degradation

14.2K

Area of Science:

  • Biotechnology
  • Environmental Science
  • Materials Science

Background:

  • Plastic, particularly polyethylene terephthalate (PET), is a ubiquitous material with a significant environmental impact due to waste accumulation.
  • Current recycling methods for PET include thermal, mechanical, and chemical processes, but biological degradation offers a novel approach.
  • The recalcitrant nature of PET necessitates innovative solutions for effective waste management.

Purpose of the Study:

  • To review the current state of knowledge regarding enzymatic degradation of PET.
  • To discuss advancements in protein engineering for enhancing PET-degrading enzymes.
  • To explore the potential of enzymatic degradation in establishing a circular plastic economy.

Main Methods:

  • Literature review of scientific publications on enzymatic PET degradation.
  • Analysis of protein engineering strategies applied to PET-degrading enzymes.
  • Discussion of the implications for sustainable plastic waste management.

Main Results:

  • Several enzymes have been identified with the capability to degrade PET.
  • Protein engineering has shown promise in improving the efficiency and stability of these enzymes.
  • Enzymatic degradation presents a viable biological recycling pathway for PET.

Conclusions:

  • Enzymatic degradation of PET is a rapidly advancing field with significant potential.
  • Optimized enzymes through protein engineering are key to efficient biological recycling.
  • This technology could be crucial for achieving a circular plastic economy and mitigating plastic pollution.