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Updated: Jan 19, 2026
A Mouse Model of Intestinal Partial Obstruction
Published on: March 5, 2018
Partially hydrolyzed guar gum alleviates small intestinal mucosal damage after massive small bowel resection along
Takayuki Fujii1, Yoichi Chiba2, Haruyuki Nakayama-Imaohji3
1Department of Pediatric Surgery, Faculty of Medicine, Kagawa University, Ikenobe, Mikicho, Kitagun, Kagawa, Japan.
Purpose:
Short bowel syndrome is associated with intestinal mucosal inflammation and microbial dysbiosis, leading to intractable complications. Partially hydrolyzed guar gum (PHGG) has trophic and anti-inflammatory effects on the intestine. We investigated whether PHGG ameliorates small intestinal mucosal damage and alters the intestinal microbiota using a rat small bowel resection (SBR) model.
Methods:
Sprague Dawley rats were divided into sham operation (Sham), Sham/PHGG, SBR, and SBR/PHGG groups. On day 21, all rats were euthanized. To assess small intestinal mucosal damage, the degeneration rate was morphometrically evaluated and immunohistochemically examined using anti-CD45 antibodies. Analyses of fecal microbiota using 16S rRNA and short-chain fatty acid production were also performed.
Results:
The mucosal degeneration rate was significantly higher in the SBR group than in the Sham or SBR/PHGG groups. The number of CD45-positive cells was significantly higher in the SBR group than in the Sham, Sham/PHGG, or SBR/PHGG groups. The relative abundance of family Lachnospiraceae was significantly higher in the SBR/PHGG group than in the SBR group.
Conclusions:
PHGG administration alleviated small intestinal mucosal damage which could be associated with modulation of the intestinal microbiota.
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