Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre

Area of Science:

• Virology
• Genomics

Background:

• Herpes simplex virus type 1 (HSV-1) is a common human pathogen.
• The MacIntyre strain of HSV-1 exhibits a significant defect in anterograde spread post-nuclear replication.

Purpose of the Study:

• To investigate the genetic basis of the MacIntyre strain's unique phenotypic characteristics.
• To identify the specific genetic determinants responsible for the observed spread defect.

Main Methods:

• Whole-genome sequencing of the HSV-1 MacIntyre strain.
• Utilized PacBio single-molecule real-time (SMRT) sequencing technology.
• Resolved the complete genome sequence of the MacIntyre strain.

Main Results:

• The complete genome sequence of the HSV-1 MacIntyre strain was successfully resolved.
• The sequencing data provides a foundation for identifying genetic variations linked to the spread defect.

Conclusions:

• The complete genome sequence of HSV-1 MacIntyre strain is now available.
• This resource will facilitate future research into the genetic determinants of viral spread defects.