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Related Experiment Video

Updated: Jan 19, 2026

Recording Brain Electromagnetic Activity During the Administration of the Gaseous Anesthetic Agents Xenon and Nitrous Oxide in Healthy Volunteers
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Anesthetic Agents Isoflurane and Propofol Decrease Maximal Ca2+-Activated Force and Thus Contractility in the Failing

Tao Meng1, Xianfeng Ren1, Xinzhong Chen1

  • 1Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan, Shangdong, China (T.M., J.Y.); Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, China (X.R.); Department of Cardiac Surgery, Tongji University Medical Center, Wuhan, China (X.C.); Division of Cardiology (J.A., N.P.) and Department of Anesthesiology and Critical Care Medicine (W.D.G.), Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; and Department of Biomedical Sciences, University of Padova, Padova, Italy (N.P.).

The Journal of Pharmacology and Experimental Therapeutics
|September 14, 2019
PubMed
Summary

Common anesthetics like isoflurane and propofol reduce heart muscle contractility in heart failure by decreasing myofilament responsiveness to calcium (Ca2+). High propofol doses also lower Ca2+ transient amplitude.

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Area of Science:

  • Cardiology
  • Anesthesiology
  • Physiology

Background:

  • Anesthetics like isoflurane and propofol affect cardiac contractility.
  • Their impact on failing myocardium remains unclear.
  • Heart failure is a significant clinical concern.

Purpose of the Study:

  • To investigate the effects of isoflurane and propofol on cardiac contractility in heart failure.
  • To elucidate the underlying mechanisms of anesthetic-induced contractility changes.

Main Methods:

  • Used rat models of monocrotaline-induced heart failure.
  • Measured force and intracellular calcium ([Ca2+]i) in isolated cardiac trabeculae.
  • Administered varying concentrations of isoflurane and propofol.
  • Performed fiber-skinning experiments to assess myofilament function.

Main Results:

  • Both anesthetics dose-dependently reduced force in failing hearts without altering [Ca2+]i at low/moderate doses.
  • High-dose propofol decreased [Ca2+]i transient amplitude.
  • Isoflurane and propofol impaired maximal Ca2+-activated force (Fmax) and increased Ca50, indicating reduced myofilament responsiveness to Ca2+.
  • No changes in cooperativity or myofibrillar ATPase-Ca2+ relationship were observed.

Conclusions:

  • Isoflurane and propofol impair contractility in failing myocardium by reducing myofilament Ca2+ responsiveness.
  • Propofol may also affect Ca2+ transient amplitude at high doses.
  • Findings have implications for anesthetic management in heart failure patients.