Recurrent non-canonical histone H3 mutations in spinal cord diffuse gliomas

Here, we present a protocol for the application of diffusion tensor imaging parameters to evaluate spinal cord...

The goal of this protocol is to obtain high-quality diffusion weighted magnetic resonance imaging (DWI) of the rat spinal cord for noninvasive characterization of tissue microstructure. This protocol describes optimizations of the MRI sequence, radiofrequency coil, and analysis methods to enable DWI images free from...

Source: Zheng, W. et. al., Diffusion Tensor Magnetic Resonance Imaging in Chronic Spinal Cord Compression. J. Vis. Exp. (2019)This video demonstrates diffusion tensor imaging in assessing chronic spinal cord compression. By analyzing water diffusion patterns, fractional anisotropy, and apparent diffusion coefficient values, the findings reveal axonal damage in compressed regions. The findings provide critical insights into spinal cord pathology and structural...

The purpose of this article is to provide a comprehensive, systematic guide to the efficient purification of histones H3 and H4 and the quantification of acetylated histone...

We describe two tactile sensory testing methods for acute or chronic periods of spinal cord injury in rats. These validated procedures can detect the development and maintenance of allodynia-like...

The present protocol describes the development of a reproducible murine model of spinal cord glioma by injecting tumor cells into the intervertebral space, offering a more effective and less invasive approach for research and therapeutic...