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Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
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The Ubiquitination of Spinal MrgC Alleviates Bone Cancer Pain and Reduces Intracellular Calcium Concentration in

Yu-E Sun1, Hua-Ye Xu2, Jing Hao2

  • 1Department of Anesthesiology, Affiliated Drum Tower Hospital, Nanjing University Medical College, No. 321 Zhongshan Road, Nanjing, 210008, Jiangsu, People's Republic of China. annasun123@163.com.

Neurochemical Research
|September 14, 2019
PubMed

Insights

Mas-related G-protein-coupled receptor subtype C (MrgC) ubiquitination is crucial for bone cancer pain in mice. This process influences intracellular calcium levels in spinal neurons, offering potential therapeutic targets for pain management.

Area of Science:

  • Neuroscience
  • Oncology
  • Pain Research

Background:

  • Mas-related G-protein-coupled receptor subtype C (MrgC) is implicated in bone cancer pain.
  • Ubiquitination is a known factor in pain signaling pathways.
  • The specific role of MrgC ubiquitination in bone cancer pain requires investigation.

Purpose of the Study:

  • To investigate the role of Mas-related G-protein-coupled receptor subtype C (MrgC) ubiquitination in the development and maintenance of bone cancer pain.
  • To explore the relationship between MrgC ubiquitination, intracellular calcium concentration, and pain behaviors in a mouse model of osteosarcoma.

Main Methods:

  • Osteosarcoma cells were implanted in mice to induce bone cancer pain.
  • Intrathecal administration of an MrgC agonist (BAM 8-22) or antagonist (anti-MrgC antibody) was performed.
  • Pain behaviors, MrgC ubiquitination levels, and spinal neuron intracellular calcium concentrations were measured.

Main Results:

  • Tumor-bearing mice exhibited significant bone cancer pain, elevated MrgC ubiquitination, and increased spinal neuron intracellular calcium.
  • MrgC agonist treatment alleviated pain, increased ubiquitination, and decreased calcium levels.
  • The effects of the agonist were reversed by the antagonist.

Conclusions:

  • MrgC ubiquitination plays a significant role in the generation and persistence of bone cancer pain in mice.
  • This process may involve the modulation of intracellular calcium concentration in spinal neurons.
  • Targeting MrgC ubiquitination presents a potential strategy for managing bone cancer pain.
Keywords:
Bone cancer painCalcium concentrationMas-related G-protein-coupled receptor subtype C (MrgC)Ubiquitination

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