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Related Concept Videos

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology11:20

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Obtaining high-quality genomic DNA from tumor tissues is an essential first step for analyzing genetic alterations using next generation sequencing. In this article, we present a simple and rapid method to enrich tumor cells and obtain intact DNA from touch imprint cytology...
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Related Experiment Video

Updated: Jan 19, 2026

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
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Rapid Lump Examination (RLE) - a bedside 3-dimensional microscopy of tumor specimens.

Nina Peters1, Melanie Schubert2, Juergen Bauer1,3

  • 1Department of Dermatology, University Medical Center Tuebingen, Tuebingen, Germany.

Journal Der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG
|September 14, 2019
PubMed
Summary

Rapid lump examination (RLE) offers a quick method for detecting basal cell carcinoma (BCC) during surgery. While promising for Mohs surgery, RLE requires further improvement for optimal diagnostic accuracy.

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Area of Science:

  • Dermatology
  • Surgical Pathology
  • Oncology

Background:

  • Rising skin cancer incidence necessitates rapid, reliable, and cost-effective therapeutic options.
  • Previous research indicates fresh tumor tissue can be microscopically examined with high accuracy.

Purpose of the Study:

  • To compare the diagnostic accuracy of rapid lump examination (RLE) for basal cell carcinoma (BCC) detection during micrographic surgery.
  • To evaluate RLE against the gold standard of formalin-fixed paraffin-embedded hematoxylin and eosin (HE)-stained sections.

Main Methods:

  • 382 specimens from 118 excised samples suspected of BCC were analyzed.
  • A standardized 60-second staining protocol was used for RLE on fresh tissue.
  • Fresh tissue samples were examined directly using a stereomicroscope.

Main Results:

  • Rapid lump examination (RLE) demonstrated high sensitivity (76%) and specificity (91%) for BCC detection.
  • Diagnostic accuracy of RLE was found to be insufficient compared to the gold standard HE-stained sections.
  • The experience of the examiner significantly influenced the results of RLE.

Conclusions:

  • RLE is a rapid and simple technique for basal cell carcinoma microscopically controlled surgery (MCS).
  • RLE shows potential to expedite workflows in Mohs surgery but requires further refinement.
  • Training and examiner experience are critical factors for successful RLE implementation.