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Updated: Jan 19, 2026

Methodology for Accurate Detection of Mitochondrial DNA Methylation
Published on: May 20, 2018
Circulating Cell Free Nuclear DNA, Mitochondrial DNA and Global DNA Methylation: Potential Noninvasive Biomarkers for
Heba A Pasha1, Noha A Rezk1, Mohamed A Riad2
1Medical Biochemistry Department, Faculty of Medicine, Zagazig University , Zagazig , Egypt.
Abstract:
Eighty seven women with benign breast lesion, 120 patients with breast cancer (BC) and one hundred controls were included in the study. Quantification of mtDNA and nDNA was done by qPCR. Global DNA methylation was measured using ELISA. Circulating cell-free nDNA and mtDNA were significantly elevated in BC and benign breast lesions patients. Global methylation was significantly low in BC patients. Combining the studied parameters in one panel, nDNA/mtDNA/hypomethylation, improved their sensitivity in detecting BC to reach 92.5%. Circulating cell-free nDNA, mtDNA and global DNA hypomethylation can be used as diagnostic and prognostic markers for BC.
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